TUGAS FARMAKOTERAPI INFEKSI dan TUMOR

UJI KEPEKAAN BAKTERI Escherichia coli
HASIL ISOLASI DARI URIN PASIEN RUMAH SAKIT Dr. Sardjito
TERHADAP ANTIBIOTIK GOLONGAN β-LAKTAM
INTISARI
Pengggunaan antibiotik yang terus menerus dan tidak terkendali dengan baik
dikhawatirkan akan meningkatkan terjadinya strain bakteri yang resisten terhadap
antibiotik. Perlu dilakukan pengujian terhadap masing-masing antibiotik untuk
melihat kepekaan bakteri E. coli terhadap antibiotik β-laktam. Uji kepekaan diawali
dengan melakukan isolasi bakteri dari sampel urin pasien di rumah sakit Dr Sardjito
dilanjutkan dengan menghitung jumlah koloni/angka kuman yang tumbuh kemudian
dilakukan identifikasi bakteri. Langkah selanjutnya dilakukan uji kepekaan bakteri
dengan metode difusi cara Kirby Bauer, dengan disk antibiotik standar ampisilin 10
μg, sulbaktam/ampisilin 20 μg, sefotaksim 30 μg, seftazidim 30 μg, seftriaxon 30
μg, sefpirom 30 μg, sefepim 30 μg dan imipenem 10 μg. Untuk masing-masing
antibiotik uji kemudian diukur diameter zona
hambatannya selanjutnya
dibandingkan terhadap tabel interpretasi zona diameter standar. Hasil penelitian
menunjukkan bahwa semua E. coli resisten terhadap antibiotik ampisilin, 65%
resisten terhadap antibiotik sulbaktam/ampisilin, 50% masih sensitif terhadap
antibiotik sefalosporin generasi ke-3 yaitu sefotaksim, seftazidim, seftriakson; lebih
dari 50% sensitif terhadap antibiotik sefalosporin generasi ke-4 seperti sefepim dan
sefpirom; dan semua bakteri E. coli sensitif terhadap antibiotik imipenem.
Kata Kunci : E. coli, Antibiotik golongan β-laktam, Uji kepekaan, Urin pasien rumah
sakit.
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THE SENSITIVITY ASSAY OF THE BACTERIA Escherichia coli
ISOLATED FROM PATIENTS URINE Dr. SARDJITO HOSPITAL
TOWARD THE β-LACTAM ANTIBIOTIC
ABSTRACT
Uncontrolled use of antibiotic caused the emergence of bacteria strain
resistant toward of antibiotic. The sensitivity assay of E. coli toward β-lactam
antibiotic has been done. The sensitivity assay was started with isolated bacteria from
patients urine at Dr. Sardjito hospital followed by count total of colony/number of
germ which growth and identified the bacteria. The Kirby Bauer method was
performed. Antibiotic impregnated disk (ampicillin 30 μg, sulbactam/ampicillin 20
μg, cefotaxim 30 μg, ceftazidime 30 μg, ceftriaxon 30 μg, cefpirom 30 μg, cefepim
30 μg and imipenem 10 μg) were placed on agar plate previously streaked with
suspensia of E. coli (1.108 CFU/ml). The plates were incubated for 18-24 hours at
370 C. The diameters of the zone inhibition were measured, and compared to standar
interpretive zone sizes. The result of the study showed that all of E. coli resistant
toward antibiotic ampicillin; 65% resistant toward antibiotic sulbactam/ampicillin;
50% still sensitive toward antibiotic third cefalosporin generation that is cefotaxim,
ceftazidime, ceftriaxon; more than 50% sensitive toward fourth cefalosporin
generation like cefepim, cefpirom and all of pathogen bacteria E. coli sensitive
toward antibiotic imipenem.
Key Words : E. coli, β-lactam antibiotic, Sensitivity assay, Urine of patient hospital.
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