PENGARUH KADAR PROTEIN DAN ENERGI METABOLIS PAKAN YANG DISUPLEMENTASI VITAMIN E DAN C TERHADAP KINERJA PRODUKSI, KUALITAS, WARNA DAN AROMA DAGING ITIK MANILA INTISARI Elly Tugiyanti 11/324283/SPT/00142 Daging Itik Manila berkualitas harus memenuhi syarat secara kualitas fisik, kimia dan organoleptik. Tujuan penelitian tahap 1: mengkaji kadar protein dan energi metabolis pakan yang mampu menghasilkan kinerja produksi (bobot badan, konversi pakan, persentase karkas dan lemak abdomen), kualitas daging (pH, daya ikat air, keempukan dan susut masak), diameter serabut otot, kadar protein, lemak dan asam lemak daging itik Manila umur 9 minggu. Rancangan yang digunakan adalah Rancangan Acak Lengkap dengan perlakuan kadar protein dengan energi pakan (R) sebagai berikut R1 = protein 13%, EM 2300 kcal/kg; R2 = protein 15%, EM 2500 kcal/kg; R3 = protein 17%, EM 2700 kcal/kg; R4 = protein 19%, EM 2900 kcal/kg dan R5 = protein 21%; EM3 100 kcal/kg. Hasil analisis sidik ragam menunjukkan bahwa perlakuan berpengaruh sangat nyata (P<0,01) terhadap bobot badan, konversi pakan, persentase lemak abdomen, lemak daging tanpa kulit, pH daging, DIA, susut masak daging, keempukan dan diameter serabut otot. Perlakuan berpengaruh nyata (P<0,05) terhadap persentase karkas, kadar lemak daging dengan kulit. Asam oleat (C18:1) paling banyak terkandung dalam daging. Tujuan penelitian tahap 2: mengkaji kadar vitamin E dan C yang disuplementasikan ke dalam pakan terhadap kinerja (bobot badan, konversi pakan, persentase karkas dan abdomen), kualitas fisik daging (pH, DIA, keempukan dan susut masak), warna (kadar mioglobin, Fe dan nilai warna), aroma daging (nilai peroksida, kadar TBARS dan TMA), asam lemak, senyawa volatil dan uji organoleptik daging itik Manila. Perlakuan terdiri EoC0 : pakan basal tanpa diberi vitamin E dan vitaminC ,E400 : pakan basal + vitamin E 400 IU, E600: pakan basal + vitamin E 600 IU;C400 : pakan basal + vitamin C 400 mg/kg pakan; C600: pakan basal +vitamin C 600 mg/kg pakan; (E200C200): pakan basal + vitamin E 200 IU+ vitamin C 200 mg /kg pakan ; dan (E300C300): pakan basal + vitamin E 300 IU + vitamin C 300 mg/kg pakan. Hasil analisis sidik ragam menunjukkan bahwa perlakuan berpengaruh sangat nyata (P<0,01) terhadap pH daging, daya ikat air, susut masak, keempukan, kadar mioglobin, Fe daging, nilai L*, nilai peroksida, TBARS,TMA dan akseptabilitas konsumen. Asam stearat (C18:0) paling banyak terkandung dalam daging. Terdapat 17 senyawa volatil golongan alkana, piran, benzen, aldehid, karboksilat dan asam akrilat penyebab aroma dan off odor daging. Kesimpulan kadar protein 21% dan energi metabolis 3100 kcal/kg pakan menghasilkan kinerja produksi, kualitas fisik daging baik, perlemakan serta diameter serabut otot yang meningkat. Suplementasi vitamin E dan C serta kombinasinya mempertahankan kinerja produksi itik Manila. Suplementasi vitamin E 300 IU dan C 300 mg memperbaiki kualitas fisik, warna, aroma secara kimia dan organoleptik, meningkatkan akseptabilitas konsumen serta menurunkan kadar alkana dan piran daging itik Manila. Kata kunci : Itik Manila, Vitamin, Off-odor, Mioglobin, Organoleptik xviii EFFECT OF PROTEIN LEVEL AND METABOLIC ENERGY OF FEED SUPPLEMENTED WITH VITAMIN E AND C ON QUALITY, COLOR AND AROMA OF MUSCOVY DUCK MEAT ABSTRACT Elly Tugiyanti 11/324283/SPT/00142 The meat of Muscovy ducks are required to meet characteristics of physical quality, chemicals content and sensory test. Stage 1 was aimed to study protein level and metabolic energy to produce the performance (final body weight, carcass weight, feed conversion, percentage of carcass and abdominal fat), meat quality (pH, WHC, tenderness and cooking loss), diameter of muscle fiber and fat and fatty acid of 9-week-old native Muscovy ducks. Research stage 1 was subject to Completely Randomized Design with protein level and metabolic energy treatments (R) consisting of R1 = protein 13%, ME 2300 Kcal/kg; R2 = protein 15%, ME 2500 kcal/kg; R3 = protein 17%, ME 2700 kcal/kg; R4 = protein 19%, ME 2900 kcal/kg dan R5 = protein 21%; ME 3100 kcal/kg. Analysis of variance showed that the treatment significantly affected (P<0.01) carcass weight, feed conversion, percentage of abdominal fat, fat content of skinless, pH, WHC, cooking loss, tenderness and diameter of meat muscle fiber. Treatment shows significant effect (P<0.05) on carcass percentage, fat content of meat with skin. Oleic acid (C18:1) was the prevalent fatty acid in meat. Stage 2 was aimed to study the level of vitamin E and C supplemented in feed on performance (body weight, feed conversion, percentage of carcass and abdominal fat), meat quality (pH, WHC, tenderness and cooking loss), color (level of myoglobin, Fe and color value), meat aroma (peroxide value, TBARS and TMA), fatty acid level, volatile organic compounds and organoleptic of meat. Treatments comprised supplementation of vitamin E, vitamin C and their combined into basal feed consisted of EoC0 : basal feed without vitamin E dan vitamin C, E400 : basal feed + 400 IUvitamin E; E600: basal feed + 600 IUvitamin E; C400 : basal feed + 400 mg/kg feed vitamin C; C600: basal feed + 600 mg/kg feed vitamin C; E200C200 : basal feed + 200 IU vitamin E + 200 mg /kg feed vitamin C; and E300C300: basal feed + 300 IU vitamin E + 300 mg /kg feed vitamin C. Analysis of variance stage 2 demonstrated that treatment highly significant effect (P<0.01) on meat pH, WHC, cooking loss, tenderness, level of myoglobin, Fe, L* value, peroxide value, TBARS, TMA level and consumers’ preference. stearic acid (C18:0) was the prevalent fatty acid in meat. There were 17 volatile compounds that categorized into alkane, pyran, benzene, aldehyde, carboxylate and acrylate acids responsible for aroma and off-odor of Muscovy duck meat. It concluded that feed containing 21% protein and 3100 kcal/kg metabolic energy could support performance, meat physical quality, fattening and muscle fiber size. Vitamin E and C supplementation supporting on Manila duck performance. Vitamin E 300 IU and vitamin C 300 mg was proven to improve meat quality, color and aroma of Muscovy duck meat either through chemical or organoleptic test, improve consumers’ preference and reduce alkana and pyran level of muscovy duck meat. Key words: Manila duck, Vitamin, Off-odor, Myoglobin, Organoleptic xix