uji potensi actinomycetes sebagai penghasil antibiotika yang

ADLN Perpustakaan Universitas Airlangga
UJI POTENSI ACTINOMYCETES SEBAGAI PENGHASIL
ANTIBIOTIKA YANG DIISOLASI DARI TANAH RHIZOSFER
MANGROVEWONOREJO SURABAYA
Fatiqin, Awalul
Pembimbing : Dr. Ir. Tini Surtiningsih, DEA
ANTIBIOTICS; ACTINOMYCETES
KKC KK TB 04 / 11 Fat u
Copyright© 2011 by Airlangga University Library Surabaya
ABSTRAK
Penelitian ini bertujuan untuk mengeksplorasi actinomycetes dari tanah rhizosfer
mangrove dan menguji potensi antibakteri serta mengetahui jenis antibiotik yang
dihasilkan. Sampel tanah diambil dari rhizosfer mangrove Wonorejo pada 5 spesies
mangrove dan daerah sampling yang berbeda. Uji aktivitas antibakteri mengunakan
metode disk modifikasi dengan bakteri uji bakteri Gram negatif (Escherichia coli ) dan
Gram positif (Staphylococcus aureus ) yang diperoleh dari Laboratorium Mikrobiologi
Fakultas Sains dan Teknologi Universitas Airlangga. Identifikasi isolat actinomycetes
dilakukan dengan mengamati karakter makroskopis koloni, mikroskopis konidia, sel
bakteri dan uji fisiologi untuk mengetahui spesies. Identifikasi jenis antibiotik
menggunakan kromatrografi lapis tipis (KLT) dan Spektrofotometer infra merah (IR).
Hasil penelitian menunjukkan bahwa didapatkan 9 isolat actinomycetes yang berbeda, 7
isolat actinomycetes mempunyai kemampuan penghambatan yang berbeda. tiga isolat
hanya menghambat pertumbuhan bakteri S. aureus, satu isolat hanya menghambat bakteri
E. coli, dan 3 isolat menghambat pertumbuhan bakteri S. aureus dan E. coli. Isolat
actinomycetes paling potensial yang dapat menghambat pertumbuhan bakteri uji adalah
spesies Streptomyces lavendulae (Ht5). Jenis antibiotik yang dihasilkan Streptomyces
lavendulae (Ht5) mempunyai kemiripan dengan antibiotik streptothricin.
Kata kunci : Actinomycetes, tanah rhizosfer mangrove, antibiotika, Streptomyces
lavendulae, Streptothricin.
ABSTRACT
This research aims was to explore actinomycetes from mangrove rhizosfer soil and
testing a potential antibacteria as knowing the types of antibiotic produced. Samples soil
were taken from mangrove rhizosfer in Wonorejo on 5 mangrove species and different
sampling areas. Test of antibacterial activities used a method of disk modification with
bacterial test which consists of negative Gram bacteria (Escherichia coli) and positive
Gram (Staphylococcus aureus) from the Laboratory of Microbiology Faculty of Sciences
and Technology, Airlangga University. Identification of actinomycetes isolates was
conducted by observing macroscopic characteristic of the colony, microscopic of conidial
Tesis
UJI POTENSI ACTINOMYCETES .....
Fatiqin, Awalul
ADLN Perpustakaan Universitas Airlangga
and bacterial cell, it was continued by physiological test to determine the species.
Identification of antibiotics types used Thin Layer of Chromatography (TLC) and
Spectrophotometer Infra-Red (IR). The result of this research obtained 9 different isolates
actinomycetes, 7 isolates actinomycetes had different inhibition ability. Three isolates
were only able to inhibits bacterial growth of S. aureus, one isolates was only able to
inhibits E. coli, and 3 isolates were able to inhibits bacterial growth of S. aureus and E.
coli. The most potential isolates actinomycetes which able to inhibit the growth of
bacterial test was a species of Streptomyces lavendulae (Ht5). Type of antibiotics that use
produced by Streptomyces lavendulae (Ht5) has a similarity with streptothricin
antibiotics.
Keywords : Actinomycetes, mangrove rhizosfer soil, Antibiotics, Streptomyces
lavendulae, Streptothricin.
Tesis
UJI POTENSI ACTINOMYCETES .....
Fatiqin, Awalul