intisari uji kepekaan bakteri methicillin-resistant

xiii
INTISARI
UJI KEPEKAAN BAKTERI METHICILLIN-RESISTANT
STAPHYLOCOCCUS AUREUS TERHADAP ANTIBIOTIK
VANCOMYCIN DENGAN METODE MACROBROTH DILUTION
Primadhy Rahardian Wijaya*, Titik Nuryastuti**, Praseno**
* Mahasiswa Fakultas Kedokteran Universitas Gadjah Mada
**Bagian Mikrobiologi, Fakultas Kedokteran Universitas Gadjah Mada
Latar Belakang : Methicillin-Resistant Staphylococcus aureus
(MRSA) merupakan bakteri patogen utama penyebab infeksi.
Infeksi yang disebabkan oleh bakteri Methicillin-Resistant
Staphylococcus aureus bersifat parah dan sulit diterapi
karena bakteri ini resisten terhadap segala antibiotik beta
laktam serta kerentanaanya untuk resisten terhadap bermacam
antibiotik. Bahkan dapat ditemui beberapa strain yang
resisten terhadap antibiotik Vancomycin yang menjadi pilihan
pertama dalam terapi bakteri ini. Penelitian ini dilakukan
untuk mengetahui kepekaan bakteri MRSA di Yogyakarta
terhadap antibiotik Vancomycin.
Tujuan : Mengetahui kepekaan bakteri Methicillin Resistant
Staphylococcus aureus terhadap antibiotik Vancomycin secara
In vitro dengan metode macrobroth dilution.
Metode : Penelitian ini berjenis penelitian observasional.
Subjek penelitian adalah antibiotik vancomycin dan bakteri
uji Methicillin-Resistant Staphylococcus aureus koleksi
laboratorium mikrobiologi Fakultas Kedokteran Universitas
Gadjah Mada. Kadar Hambat Minimum (KHM) dan Kadar Bunuh
Minimum
(KBM)
untuk
Vancomycin
terhadap
MethicillinResistant Staphylococcus aureus diperiksa dengan menggunakan
Metode macrobroth dilution dan dicocokkan sesuai standar
CLSI.
Hasil :
Kadar
Hambat
Minimum (KHM) dari 5
isolat
Methicillin-Resistant
Staphylococcus
aureus
yang
diuji
kepekaannya terhadap antibiotik Vancomycin, 4 dari 5 isolat
(80%) dinyatakan sensitif terhadap vancomycin. Sebanyak 1
dari 5 isolat (20%) dinyatakan intermediate dan 0 dari 5
isolat (0%) dinyatakan resisten. Kadar Bunuh Minimum (KBM)
dari Vancomycin menunjukkan efek bakterisidal terhadap
bakteri MRSA.
Kesimpulan :Bakteri
Methicillin-Resistant
Staphylococcus
aureus masih cukup sensitif terhadap antibiotik vancomycin.
Kata Kunci :Vancomycin, Methicillin-Resistant Staphylococcus
aureus (MRSA), Kadar Hambat Minimum (KHM), Kadar Bunuh
Minimum, Kepekaan, Sensitivitas, Antibiotik.
xiv
ABSTRACT
SENSITIVITY TEST OF METHICILLIN-RESISTANT
STAPHYLOCOCCUS AUREUS AGAINST VANCOMYCIN WITH
MACROBROTH DILUTION METHOD
Primadhy Rahardian Wijaya*, Titik Nuryastuti**, Praseno**
* Undergraduate Student of Faculty of Medicine, Gadjah Mada
University
** Department of Microbiology, Faculty of Medicine, Gadjah Mada
University
Background : Methicillin-Resistant Staphylococcus aureus is
one of main pathogen causing infection in human beings.
Methicillin-Resistant Staphylococcus aureus infections is
often severe and hard to treat because it’s capability to
resists any beta lactams antibiotics and its fast ability to
develop resistant against various antibiotics. Even, some of
strains are found to be resistant against Vancomycin that
has been a drug of choice to treat this bacterial infection.
This research’s objective is to know the sensitivity of MRSA
to antibiotic vancomycin in Yogyakarta.
Objectives
:
To
know
Vancomycin
sensitivity
against
Methicillin Resistant Staphylococcus aureus with macrobroth
dilution method.
Method : This study used observational design. The materials
were
Vancomycin
antibiotic
and
Methicillin-Resistant
Staphylococcus aureus isolate collections of Laboratorium of
Microbiology Faculty of Medicine Universitas Gadjah Mada.
Minimal
Inhibitory
Concentration
(MIC)
and
Minimal
Bacteriocidal Concentration (MBC) of Vancomycin against
Methicillin-Resistant Staphylococcus aureus were examined
using Macrobroth Dilution and match it with CLSI standard.
Results : Minimal Inhibitory Concentration (MIC) of 5
Methicillin-Resistant Staphylococcus aureus isolates tested
against Vancomycin were 80% sensitive, 20% intermediate, and
0% resistant.
Conclusion : Vancomycin has antibacterial effect and still
sensitive
againts
Methicillin-Resistant
Staphylococcus
aureus
Key Words: Vancomycin, Methicillin-Resistant Staphylococcus
aureus (MRSA), Minimal Inhibitory Concentration (MIC),
Minimal Bacteriocidal Concentration (MBC), Sensitivity,
Antibiotic.