IDENTITAS PENULIS JUDUL Pengaruh Ekstrak Cumi

IDENTITAS PENULIS
JUDUL
Pengaruh Ekstrak Cumi-Cumi (Loligo sp) Terhadap Kadar TNF-α dan TGF-β1 Serum pada
Penyembuhan Luka Tikus Model Diabetes Mellitus
The Effect of Squid Extract (Loligo sp) Toward The Levels of TNF-α and TGF-β1 Serum for Wound
Healing of a Rat Diabetes Mellitus Model
ABSTRAK
Diabetes Mellitus (DM) adalah suatu penyakit kronis yang ditandai oleh peningkatan kadar glukosa
darah (hiperglikemia) dan dikarenakan oleh resistensi insulin atau defisiensi insulin, sehingga menyebabkan
terjadinya lambatnya proses penyembuhan luka. Produksi sitokin proinflamasi yang berlebihan, penurunan
TGF-β1, peningkatan jumlah makrofag dan neutrofil dapat menjadi salah satu karakteristik adanya
keterlambatan penyembuhan luka. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak cumi-cumi
terhadap kadar TNF-α serum, kadar TGF-β1 serum, jumlah makrofag, jumlah neutrofil, dan eritema. Penelitian
menggunakan metode Randomized Posttest Only Controlled Group Design dilakukan terhadap tikus Galur
Wistar. Hewan coba dibagi menjadi 6 kelompok berupa luka normal dan diabetes dengan diet standar,
pemberian suplemen chitosan, ekstrak cumi-cumi secara oral satu kali sehari, dua kali sehari, dan dua hari
sekali. Kadar TNFα dan TGFβ 1 diamati melalui metode ELISA, makrofag dan neutrofil menggunakan
pewarnaan H&E, eritema dihitung menggunakan program Corel Photo-Paint X6, dan dianalisis statistika
dengan menggunakan uji One-Way ANOVA. Hasil penelitian menunjukkan bahwa ekstrak cumi-cumi efektif
dalam menurunkan kadar TNF-α serum, jumlah makrofag, dan jumlah neutrofil. Namun tidak mempengaruhi
penurunan eritema dan peningkatan kadar TGF-β1 serum.
Kata kunci: luka kondisi diabetes, TNF-α, TGF-β1, cumi-cumi
ABSTRACT
Diabetes Mellitus (DM) is a chronic disease characterized by elevated levels of blood glucose (hyperglycemia)
due to impaired insulin action in glucose metabolism resulting in impaired wound healing. Excessive production
of pro-inflammatory cytokines, increased number of macrophages and neutrophils, and decrease levels of TGFβ1 serum can be the characteristics of impaired wound healing. This study aims to determine the effect of squid
extract to some wound parameters such as levels of TNF-α, and TGF- β1 serum, number of macrophages and
neutrophils, and erythema. The research designed as Posttest Only Randomized Controlled Group Design
conducted on experimentally male Wistar rats. Experimental animals were divided into 6 groups including
normal and diabetic wound with standard diet, diabetic wound with chitosan supplement, diabetic wound given
orally squid extract once a day, twice a day, and once in two days. Levels of TNF-α and TGF-β1 serum were
observed by ELISA, macrophages and neutrophils by H & E staining, erythema was calculated using Corel
Photo-Paint program X6, and analyzed statistically by One-Way ANOVA. The results indicated that squid
extract is effective in lowering levels of TNF-α, serum the number of macrophages and neutrophils. However, it
has insignificantly shown reduction of erythema and increasing levels of TGF-β1 serum.
Keywords: diabetic wound, TNF-α, TGF-β1, squid
Author’s Identity:
1.
Kadek Nova Prayadni Dewi
Nursing Program, Medical Faculty, University of Brawijaya
Address: Jalan Veteran Malang
Institute phone number: (0314) 551611
Email: [email protected]
2.
Ns. Heri Kristianto, S. Kep., M.Kep., Sp.KMB
Medical-surgical Nursing Specialist Nursing Program, Medical Faculty, University of Brawijaya
3.
Prof. DR. dr. Mochamad Rasjad Indra, MS
Department of Physiology Medical Faculty, University of Brawijaya