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ALKALOID

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ALKALOID
INDOL
SIMPLE
INDOLES
FASE DIAM
Silika gel G
FASE GERAK
Kloroform,
toluene
Aseton,
methanol,
etanol
Ethyl acetate,
propan-2-ol,
and 13.5 M
ammonia, i.e.,
100:2:1,
16:3:1, and
9:7:4
Methanol:0.2
M ammonium
nitrate
silica gel
methanol:13.5 M
ammonia 100:1.5
UV
first
observing
under UV
light
254 nm for
the presence
of fluorescent
or quenching
zones.
If the latter
are sought,
then a layer
with added
fluorophor
(e.g., silica
gel GF
) should be
used. The
Cinchona
alkaloids in
particular
give a very
strong bright
blue
fluorescence
under
UV 365 nm
and can be
detected at
very low
concentration
s. Many of
the ergot
alkaloids
give a blue
fluorescence
under UV
light at both
254 and 365
nm.
REAGEN
Dragendorff’s
Reagent, Gives an
orange
color with many
types of
alkoloids.
4Dimethylaminobenzal
dehyde solution in an
acidic medium, such
as HCl, is
own as Ehrlich’s
reagent or Van Urk
reagent, and gives a
blue color with simple
indoles such as the
harmane series, and
particularly with the
ergot alkaloids. It has
been extensively used
to detect the alkaloids
of ergot with which it
gives blue, violet,
or green colors.
Cerium(IV) sulfate
solution gives a
variety
of colors with the
Catharanthus and
Vinca alkaloids,
18,19
while iron(III)
chloride=
perchloric acid has
been widely utilized
for many indole and
oxindole compounds,
especially those from
Rauvolfia, Strychnos,
Mitragyna,andUncaria
with which a wide
variety of colors are
seen.
dragendorff’s
o-phthalaldehyde=sulfuric
acid and this can detect
amounts
as low as 20 ng for
ERGOT
ALKALOI
silica gel
toluene:chlorofor
m:ethanol
28.5:57:14.5
formamide:ethano
l 8:15
acetone:0.1 M
ammonium
carbonate:ethanol
32.5:67:5.1
Silica gel
diethylamine
13.5 M
ammonia
DS
TERPEN
OIDAL
INDOLE
ALKALOI
UV light 254
and 365
nm, and as blue
zones
iron(III) chloride
solution=perchloric
acid, and also ceric
sulfate spray
DS
QUINO
LINE
ALKAL
OIDS
variety of colors with
different types of
alkaloids
Silica gel
INDOL
ES
WITH A
QUATE
RNARY
NITRO
GEN
OTHER silica gel
INDOL
E
ALKAL
OIDS
indoloqui
nolizidin
serotonin and 100 ng for
bufotenine.
which change to a purple
color on heating, with Van
Urk reagent.
Iodoplatinate reagent gives
a
range of colors which
enable the common
alkaloids to be
distinguished from one
another, for example,
ergometrine gives a white
zone against a pinkish
background
while ergotamine gives a
strong pink color and
ergocristine a pale brown.
0% of a base
(e.g.,
diethylamine in
a weakly polar
solvent such
as chloroform)
UV 365 nm
dark violet
fluorescence.
0.2 M aqueous
ammonium
nitrate to
methanol (2:3)
ethyl
acetate:formic
acid:water
6:1:1
chloroform:metha
nol:water 7:3:1
chloroform:metha
bright yellow
fluorescent
compound
under UV
light 365 nm
With iodoplatinate
reagent the four
compounds give
slightly different colors
with
quinine giving violetbrown, cinchonidine
grey-violet, quinidine
red-violet, and
cinchonine
brown-red.
46
e
alkaloid
cryptolep
ine
Canthino
ne
Camptot
hecin
Ellipticin
e
nol 97:3
benzene:chlorofor
m:ethanol
Alkaloid Isoquinolon
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