Malaria Microscopy Training Center Waikabubak, Sumba This is to certify that _________________________________________________________ has successfully completed malaria microscopy training and assessment of malaria preparation and reading skills with the following scores: Sensitivity: _____%, Specificity: _____%, Species Accuracy: _____%, Counting Accuracy: _____%, Slide Prep:_____% Qualified as:___________________ Malaria Microscopist, Certificate No: MTC-2018-_____ Examination date: __________ 2018, Valid until: __________ 2020 (2 years) Kepala Dinas Kesehatan Health Program Director Drg. Bonar B. Sinaga, M.Kes. Pembina Utama Muda NIP. 19680220 199312 1 002 Dr. Claus Bogh Ketua The Sumba Foundation Malaria theory, diagnosis, treatment and control • The Epidemiology and biology of the malaria parasite • Clinical malaria and treatment of malaria cases This malariasafety microscopy training is developed according to WHO • Personal and good clinical practice • Mass blood surveys at village level and clinic level malaria screening • Malaria eradication through parasite elimination and use of mosquito net • Malaria species and parasite stage recognition • Malaria parasite counting • Malaria case reporting in the Government Health System • Education in the anti corruption policies of Indonesia Slide reading • Accurately identify positive and negative malaria blood film • Accurately identify the different species of Plasmodium • Accurately identify mixed infections of Plasmodium • Accurately identify the different stages of malaria parasites • Accurately count the different species and stages of parasites Microscopy • Prepare excellent thick and thin malaria blood films • Collection of blood samples in villages/health centers/hospitals and guidelines and covered following • National Correct dilution and use of prepared stockthe of Giemsa stain topics: and buffer • Correct straining of malaria blood films. • Correctly set up a microscope (incl. correct illumination) • Correctly use immersion oil. • Basic cleaning/maintenance of microscope • Filariasis, drug affected malaria parasites and artifact identification Further general theory during training • Good patient handling practice for malaria diagnosis • Government cross checking system • Patient information ethics and handling Duration of standard training: 4 weeks or 24 working days, totaling minimum 170 hours for theory, laboratory and field lessons. Below are the WHO and RI national grades for accreditation of peripheral malaria microscopists (Ref: WHO 2006 and WHO 2016). The peripheral examination set consists of 25 slides for species identification, 6 slides for parasite counting and students preparation of 5 thick and thin film slides. Each slides must be read within 10 min. The slide sets includes negative and positive slides including all 4 Plasmodium species as well as mixed infections of human plasmodium. Accreditation Level Level 1 WHO Expert Detecting positive slides (Sensitivity) 100 % Detecting negative slides (Specificity) Accuracy of Species Identification Accuracy of Preparation of thick Parasite Counting and thin blood films (within 25% of true count) 100 % >90 % (100% Pf) 50-100 % 90-100 % 50-100 % 90-100 % Level 2 National A 90-100 % 90-100 % 90-100% (>95% Pf) Level 3 National B 80-89 % 80-89 % 80-89 % 30-49 % 80-89 % In training <80 % <80 % <80 % >30 % >80 %