xii PENGARUH SPERMATOZOA BANGSA SAPI YANG

PENGARUH SPERMATOZOA BANGSA SAPI YANG
BERBEDA TERHADAP PERKEMBANGAN
EMBRIO IN VITRO
Nang Pranawa Adyaksa
13/359695/PPT/000856
INTISARI
Penelitian ini bertujuan untuk mengetahui pengaruh spermatozoa
bangsa sapi Simmental, Wagyu, Brahman dan Bali terhadap perkembangan
embrio in vitro. Ovarium diambil dari rumah potong hewan (RPH), dibawa dalam
larutan NaCl pada suhu 31 sampai 34°C ke laboratorium BET. Oosit dibagi ke
dalam empat kelompok berdasarkan spermatozoa bangsa sapi yang digunakan
untuk proses fertilisasi. Selanjutnya oosit diinkubasi selama 22 jam untuk proses
maturasi. Sperma beku dithawing kemudian dicuci lalu dimasukkan dalam
centrifuge tube, dicuci 2 kali. Sperma diinkubasi selama 5 jam untuk kapasitasi.
Oosit yang telah dimaturasi dicuci dan diinkubasi dalam drop semen untuk
proses fertilisasi selama 5 jam. Oosit yang telah difertilisasi, selanjutnya dicuci
dengan medium kultur (CR1aa) dan diinkubasi. Pengamatan perkembangan
embrio dilakukan pada hari ke-6 hingga hari ke-8. Data evaluasi embrio yang
telah diperoleh dianalisis secara deskriptif. Pengaruh sperma dari bangsa sapi
yang berbeda terhadap perkembangan embrio in vitro dianalisis dengan oneway
ANOVA. Hasil dalam penelitian menunjukan spermatozoa dari bangsa sapi yang
berbeda berpengaruh terhadap perkembangan embrio secara in vitro yaitu
Simmental (26,15 ± 7,579), Bali (41,21 ± 9,076), Brahman (40,74 ± 6,249), dan
Wagyu (32,19 ± 2,867). Perkembangan embrio terbaik diperoleh dengan
menggunakan spematozoa sapi Bali. Fertilisasi in vitro dengan menggunakan
spermatozoa sapi Bali dan sapi Wagyu menghasilkan kualitas embrio rata-rata
pada grade III, sedangkan sapi Simental dan sapi Brahman menghasilkan
kualitas embrio rata-rata pada grade IV.
Kata kunci: Spermatozoa, Bangsa sapi, Fertilisasi in vitro, Perkembangan
embrio.
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THE EFFECT OF DIFFERENT BREED CATTLE SPERMATOZOA
ON THE IN VITRO EMBRYO DEVELOPMENT
Nang Pranawa Adyaksa
13/359695/PPT/000856
ABSTRACT
This research was conducted to determine the effect of Simmental, Bali,
Brahman, and Wagyu spermatozoa on the in vitro embryo development. Ovaries
were collected from slaughter house and transportation into sodium chloride
(NaCl) solution at 31 to 34oC to BET’s laboratory. Oocytes were collected from
ovaries by aspiration method. Oocytes were incubated during 22 hour for
maturation. Oocytes were divided into four groups base on the kind of bull breed
spermatozoa. Frozen semen were thawed, flushed, and centrifuged five minutes
for washing. The semen were incubated during 5 hours for capacitazion. Matured
ocytes were flushed using oocyte flushing medium and then incubated for 5
hours in the semen drop for fertilization process. Fertilized oocytes were flushed
and incubated for two day. The embryo development was observed from day
sixth to eighth post fertilized. The data of embryo development were analyzed
descriptive. The effect of the different breed cattle spermatozoa on embryo
development analyzed using oneway ANOVA. The result showed that the
spermatozoa from different breeds had affected on vitro embryo development:
Simmental (26.15 ± 7.579); Bali (41.21 ± 9.076); Brahman (40.74 ± 6.249), and
Wagyu (32.19 ± 2.867). The best result of embryo development was showed by
Bali breed. In vitro fertilization using Bali and Wagyu sperm given the embryo
quality at the third grade, while Simmental and Brahman at the fourth grade.
Key words: Spermatozoa, Bull breed, In vitro fertilization, Embryo’s development.
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