ABSTRAK SUPLEMENTASI MINYAK IKAN UNTUK PENINGKATAN IMUNITAS NON SPESIFIK UDANG VANAME (Litopenaeus vannamei) Oleh Vina Olivia Indraswati Peningkatan imunitas udang menjadi salah satu target pemberian pakan dengan komposisi penyusun nutrien yang lengkap. Udang memiliki sistem imun yang berbeda dengan ikan pada umumnya dan dapat ditingkatkan dengan cepat dengan pemberian nutrien yang tepat. Penelitian suplementasi nutrien untuk peningkatan imunitas non spesifik pada udang khususnya pada udang vaname (Litopenaeus vannamei) masih sedikit dilakukan khususnya dengan penambahan minyak ikan. Penelitian ini dilakukan untuk mempelajari pengaruh suplementasi minyak ikan yang berbeda diantaranya minyak lemuru dan minyak patin dengan memperhatikan paremeter non spesifik udang vaname diantaranya total haemocyte count (THC), differential haemocyte count (DHC), uji tantang dengan Vibrio harveyii dan tingkat perlindungan relatif -relative percent survival (RPS). Desain percobaan menggunakan rancangan acak lengkap dengan tiga perlakuan dan empat ulangan. Perlakuannya yaitu penambahan minyak lemuru (perlakuan A), minyak patin (perlakuan B) dan tanpa penambahan minyak ikan (kontrol). Hasil penelitian menunjukkan bahwa perlakuan penambahan minyak lemuru dan minyak patin dapat meningkatkan THC, DHC, aktivitas fagositosis yang mempengaruhi tingkat perlindungan relatif dibandingkan dengan tanpa pemberian minyak ikan (P<0,05). Peningkatan imunitas non spesifik pada udang vaname dengan pemberian minyak ikan perlu dikaji analisis ekonominya agar ekonomis jika diterapkan pada tingkat petani udang atau pabrik pakan komersil. Kata kunci: Imunitas, Litopenaeus vannamei, Total Haemocyte Count, Differential Haemocyte Count. ABSTRACT DIFFERENT FISH OILS ENHANCES NON-SPECIFIC IMMUNITY OF PACIFIC WHITE SHRIMP (Litopenaeus vannamei) By Vina Olivia Indraswati Increased immunity shrimp became one of the targets of feeding with a complete nutrient constituent composition. Shrimp have a different immune system of fish in general and can be increased quickly by providing the right nutrients. Research of nutrient supplementation to increase non-specific immunity, especially in vaname shrimp (Litopenaeus vannamei) is still rarely done, especially with the addition of fish oil. This research was conducted to study the effect of fish oil supplementation in different oil among lemuru oil and patin oil with a nonspecific parameter attention vaname shrimp among total haemocyte count (THC), the differential haemocyte count (DHC), challenge test with Vibrio harveyii and relative percent survival (RPS). Design of experiments was using a completely random design with three treatments and four replications. The treatments were the adding of the lemuru oil (treatment A), patin oil (treatent B) and without the addition of fish oil (control). The results showed that the addition of lemuru fish and patin fish oil treatment could increase THC, DHC, which affected the phagocytic activity relative to the level of protection without giving fish oil (P <0.05). The increased of non-specific immunity in vaname shrimp with fish oil is needed to analyze in the economic side to be more economical if applied to the shrimp farmers or commercial feed mills. Keywords: Immunity, Litopenaeus vannamei, Total Haemocyte Count, Differential Haemocyte Count.