LAPORAN PENELITIAN HIBAH PENELITIAN STRATEGI NASIONAL TAHUN ANGGARAN 2009 EKSPRESI PROTEIN LAMUN (SEAGRASS) TERHADAP PENCEMARAN LOGAM BERAT TIMBAL (Pb) DI PERAIRAN PESISIR UTARA JAWA TIMUR OLEH : Dr.Ir.Endang Yuli Herawati., MS. Dr.drh. Aulanni’am, DES Dibiayai oleh Direktorat Jendral Pendidikan Tinggi, Departemen Pendidikan Nasional, melalui DIPA Universitas Brawijaya No 0174.0/023—04.2/XV/2009 tanggal 31 Desember 2008, dan berdasarkan SK Rektor Nomor : 160/SK/2009, tanggal 7 Mei 2009 UNIVERSTAS BRAWIJAYA MALANG, NOVEMBER 2009 RINGKASAN Resiko polusi lingkungan ion logam berat Pb karena toxisitas serta menyebabkan kelumpuhan dan akumulasi dalam biota, maka diperlukan monitoring konsentrasi logam berat dalam lingkungan. Analisa kandungan logam berat dalam sedimen dan air banyak dilakukan namun dalam tumbuhan lamun masih sangat sedikit dilakukan. Tujuan 1). Mengetahui kandungan Pb pada akar, seludang dan daun lamun, sedimen dan air dari lokasi yang berbeda 2). Menentukan tingkat pencemaran perairan pesisir Utara Jawa Timur berdasarkan kandungan logam berat Pb; 3). Mengetahui dan menentukan profil protein bagian - bagian tumbuhan lamun yang terkontaminasi Pb. Hasil penelitian didapat 1). Kandungan Pb dalam tumbuhan lamun dan sedimen di lingkungan tercemar cenderung lebih tinggi daripada di lingkungan tidak tercemar; 2). Kandungan Pb air laut Lamongan (0,062 ± 0,003 ppm) dan Lempuyang (0,049 ± 0,004 ppm) < sedimen Lamongan (5,847 ± 0,058 ppm) dan Lempuyang (1,238 ± 0,034 ppm) namun pada air sudah melebihi ambang batas Baku mutu air laut untuk kehidupan biota oleh KMNLH (2004) 0,008 ppm; 3). Profil protein menunjukkan terekspresi hampir sama dengan berat molekul 75,7 kDa di Lamongan dan 72 kDa di Lempuyang dari sampel akar, seludang dan daun lamun Cymodocea rotundata. Disarankan 1). Tumbuhan lamun berguna untuk membantu mengurangi toksisitas Pb di perairan pesisir; 2). Perlu penelitian tentang luas area tumbuhan lamun pada suatu perairan dalam upaya mengurangi kandungan Pb dan 3). Perlu dilakukan pengamatan tentang pengaruh salinitas yang lebih rendah Kata kunci : lamun dan logam berat Pb SUMMARY Environmental pollution risk Pb heavy metal ion because toxicitas and causes immobility and accumulation in biota, therefore needful monitoring concentrates heavy metal in environmentally. Heavy metal concentration analysis in sedimentary and water a lot of is done but on seagrass still measly is done. To the effect 1). Knowing Pb content on seagrass parts (root, rhizome and leaf ), sediment and water of location that variably 2). Determining pollution degree Javanese North coast East base Pb's heavy metal content; 3 ). Know and determines seagrass part protein profiles contamination Pb’s ones. Observational result to be gotten 1). Pb's content in seagrass and sedimentary at begrimed environment tends to overbid than at environment is not begrimed; 2 ). Pb's content water goes out to sea Lamongan (0,062 ± 0,003 ppm) and Lempuyang (0,049 ± 0,004 ppm) < Lamongan's sediment (5,847 ± 0,058 ppm) and Lempuyang (1,238 ± 0,034 ppm) but on water was overshot Default bounds sill water quality goes out to sea to biota's life by KMNLH (2004) 0,008 ppm; 3 ). Protein profile points out most expression close resemblance heavily molecule 75,7 kDa at Lamongan and 72 kDa at Lempuyang of root sample, seludang and leaf romances Cymodocea rotundata . Suggested 1). Seagrass plant to help reduce Pb's toxicity at coast waters; 2 ). Need research seagrass area extent at one particular waters in the effort reduce Pb's content and 3). Need to be done by more watch about inferior salinity influence Key word : Seagrass and Pb heavy metal DAFTAR PUSTAKA American Public Health Association (APHA), 1989. Standard Methods for The xamination f Water and Waste Water. 17 ed. 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