Peran Fraksi Flavonoid Sarang Semut (Myrmecodia Pendans)

ABSTRAK PENELITIAN KOMPETISI INTERNAL
TAHUN 2014
Lembaga Penelitian dan Pengabdian Masyarakat (LP2M)
Universitas Hasanuddin
Kampus Unhas Tamalanrea
Jln. Perintis Kemerdekaan KM. 10 Makassar
Telp. : 0411 587032, , 582500, 588888 Fax.(0411) 587032, 584024
Website : http://www.unhas.ac.id/lppm email : [email protected]
The Role of Sarang Semut (Myrmecodia pendans) Flavonoid’s Fraction in Proliferation
and Angiogenesis Inhibition of Human Tongue Squamous Cell Carcinoma
Harun Achmad1*, M. Hendra Chandha2, Sri Ramadhany3, Hendrastuti Handayani1,
Rasmidar Samad4
1
Lecturer, Pediatric Dentistry Department, Faculty of Dentistry, Hasanuddin University
2
Lecturer, Oral Surgery Department, Faculty of Dentistry, Hasanuddin University
3
Lecturer, Public Health Department, Faculty of Medicine, Hasanuddin University
4
Lecturer, Public Dental Health Department, Faculty of Dentistry, Hasanuddin University
*Correspondence: [email protected]
Running Title: Sarang semut flavonoid, VEGF, Interleukin-8, Angiogenesis
ABSTRACT
Background: Squamous cell carcinoma is the most common type of cancer that occurs in the
oral cavity which is about 90-95 % of total malignancy in the oral cavity. The aim of this study
was to analyze the effect of the flavonoid’s fraction of Sarang Semut (Myrmecodia pendans) as
an anti-cancer to angiogenesis inhibition by suppressing the VEGF and IL-8 expression in SP-C1
human tongue cancer cell.
Material and Method: This research was conducted with the pure laboratory experimental
method using Supri’s-Clone 1 (SP-C1) human tongue cancer cell culture. This research was
started with cytotoxicity test to obtain the fraction of flavonoid that possesses anti-cancer
potentiality and angiogenesis inhibition test.
Result: The result of this study showed that ethyl acetate and ethanol fraction of flavonoid had a
role in inhibiting the VEGF protein and interleukin 8 expressions in SP-C1 tongue cancer cell.
ANOVA test with significance level (α = 0,05) which resulted in highly significant difference in
the concentration with a mean optical density (OD) absorbance ethanol fraction (p = 0,00), ethyl
acetate fraction (p = 0,00), and controls (p = 0,00) for the suppression of VEGF protein
expression and interleukin-8 of SP-C1 human tongue cancer cell. Western blotting analysis
showed that ethyl acetate fraction of flavonoid application to SP-C1 human tongue cancer cell
showed a decrease in SP-C1 protein expression.
Conculusion: Flavonoid fraction of sarang semut inhibit the angiogenesis in SP-C1 tongue cell
cancer by suppressing the VEGF and interleukin 8 protein expression. Ethyl acetate fraction
from sarang semut’s flavonoid inhibited the expression signal transduction factor from SP-C1
tongue cancer cell.
Keywords: SP-C1 tongue squamous cell carcinoma, sarang semut flavonoid’s fraction,
angiogenesis, VEGF, interleukin-8
Peran Fraksi Flavonoid Sarang Semut (Myrmecodia Pendans) Terhadap Hambatan
Proliferasi dan Hambatan Angiogenesis pada Squamous Cell Carcinoma Lidah Manusia
ABSTRAK
Pendahuluan: Karsinoma sel skuamosa merupakan jenis kanker yang paling sering terjadi di
rongga mulut yaitu sekitar 90-95% dari total keganasan pada rongga mulut. Penelitian ini
bertujuan menganalisis pengaruh fraksi flavonoid Sarang Semut (Myrmecodia pendans) sebagai
anti kanker terhadap mekanisme hambatan angiogenesis melalui penekanan ekspresi VEGF dan
IL-8 pada sel kanker lidah manusia SP-C1.
Bahan dan Metode: Penelitian dilakukan dengan metode eksperimental murni laboratorik
dengan menggunakan biakan sel kanker lidah manusia Supri’s-Clone 1 (SP-C1). Penelitian
bertahap dimulai uji sitoksisitas untuk mendapatkan fraksinasi flavonoid dengan potensi
antikanker, hingga uji hambatan angiogenesis.
Hasil: Hasil penelitian menunjukkan flavonoid fraksi etil asetat dan etanol memiliki peran dalam
hambatan ekspresi protein VEGF dan interleukin 8 pada sel kanker lidah SP-C1. Analisis uji
ANOVA dengan tingkat kemaknaan (=0.05) menghasilkan perbedaan yang sangat bermakna
pada konsentrasi dengan rerata optical density (OD) absorbansi fraksi etanol (p = 0,00), fraksi
etil asetat (p =0,00) dan kontrol (p =0,00) terhadap penekanan ekspresi protein VEGF dan
Interleukin-8 sel kanker lidah SPC1.
Simpulan: Fraksi flavonoid sarang semut menghambat angiogenesis pada sel kanker lidah SPC1 melalui penekanan ekspresi protein VEGF dan Interleukin 8. Flavonoid fraksi etil asetat
sarang semut menghambat ekspresi protein pada sel kanker lidah SP-C1.
Kata kunci: Karsinoma sel skuamosa lidah SP-C1, Fraksi flavonoid sarang semut,
Angiogenesis, VEGF, Interleukin-8.