materi 7 clevage dan diferensiasi
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MATERI 7 PERKEMBANGAN KONSEPTUS MK. Ilmu Reproduksi LABORATORIUM REPRODUKSI TERNAK FAPET UB 1 SUB POKOK BAHASAN • Proses pembelahan sel zygote • Differensiasi sel embrio dan perkembangan embrio awal • Proses implantasi • Faktor-faktor yang mempengaruhi perjalanan embrio hingga terjadinya implantasi LABORATORIUM REPRODUKSI TERNAK FAPET UB 2 Ovulation Only fertilized ova are transported into the the uterine horn of the mare Oviduct Ovary Oviductal Transport in the Mare Unfertilized Ova Uterine Horn Blastocyst PGE Release LABORATORIUM REPRODUKSI TERNAK FAPET UB 3 Ovulasi dan Fertilisasi LABORATORIUM REPRODUKSI TERNAK FAPET UB 4 Early Embryonic Development Location All cell divisions are mitotic Smaller cell size with each cleavage division Enters uterus Even if not fertilized Except Mare - Unfertilized remain in oviduct Day Ampullary- 0-2 Isthmic Junction Ampullary- 1-3 Isthmic Junction Development One cell Two cell Isthmus 2-3 Four cell Isthmus 3-5 Eight cell Uterus 4-5 Sixteen cell Uterus 5-8 Morula LABORATORIUM REPRODUKSI TERNAK FAPET UB Two Polar Bodies Separate Blastomere’s for twins Fuse two morula together for a chimera 5 Day Development 6-7 Tight Morula 7-8 Early Blastocyst 7-9 Blastocyst Inner Cell Mass Forms Embryo 8-10 Expanded Blastocyst Contraction and expansion of blastocyst as well as enzymes are involved with zona removal 9-11 Compact Morula form tight cell contacts so that Blastocyst formation is possible Fills with fluid in center Cells have established polarity Blastocoel Trophoblast Forms Placenta If split ICM get twins Hatching Blastocyst LABORATORIUM REPRODUKSIand TERNAK Picture reproduced with permission of Academic Press In: Physiology Technology of Reproduction FAPET UB Picture courtesy of Dr. J.E. Flechon 6 Unpolarized Morula Polarized Morula Inner Cell Mass Blastocoel Blastocyst Blastocoel LABORATORIUM REPRODUKSI TERNAK FAPET UB Trophectoderm 7 LABORATORIUM REPRODUKSI TERNAK FAPET UB 8 Embrio Hatching Terjadi Implantasi (Ternak Bunting) LABORATORIUM REPRODUKSI TERNAK FAPET UB 9 Embryo sapi LABORATORIUM REPRODUKSI TERNAK FAPET UB 10 Blastocyst - Nuclei Inner Cell Mass LABORATORIUM REPRODUKSI TERNAK FAPETPhotomicrograph UB courtesy of Dr. Daniel Pomp 11 Time comparisons from ovulation during cleavage for different farm species Species Cattle 1 cell 8 Cell Blastocyst Enter Uterus Length of (hours) (Days) Gestation 24 3 8 3.5 281 Horse 24 3 6 5 Note 337entry Late in mare Sheep 24 2.5 7 3 148 Swine 14-16 2 6 2 114 LABORATORIUM REPRODUKSI TERNAK FAPET UB 12 Differentiation o f Conceptus Cell Types Inner Cell Mass 1 2 Three Layers Form 3 Day 14 Expanded LABORATORIUMBlastocyst REPRODUKSI TERNAK FAPET UB 13