Sequence: Zebrafish 7.4 lb lck promoter Gateway 5' entry clone, JY2122.dna (Circular / 10,030 bp) Enzymes: < Unsaved Enzyme Set > (33 of 646 total) Features: 10 visible, 10 total Unique Cutters Bold Created by Jeff Yoder lab, NC State University, lab ID "JY2122" (9922) DrdI BspQI - SapI (111) rrnB T2 terminator M13 fwd AhdI (549) BtgI - NcoI - StyI (581) SfiI (696) FspI (704) Bpu10I BsmBI (8849) AsiSI - PvuI (8866) (8865) (8506) NruI BsaI (1686) M13 rev (8258) EagI - NotI NdeI (8184) Zebrafish 7.4 kb lck promoter Gateway 5' entry clone 10,030 bp PpuMI (2473) SfiI (2570) HindIII (3173) (6687) PmlI (6515) MscI Printed from SnapGene®: Wednesday, Jul 09, 2014 4:48:40 PM SphI (3574) BanI - KasI (4051) NarI (4052) SfoI (4053) PluTI (4055) SexAI* (4062) XcmI (4070) AarI (4142) (4600) SpeI SalI (4477) Page 1