7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate LAPORAN PEMERIKSAAN GOLONGAN DARAH ABO DAN RHESUS OLEH KELOMPOK IV Ni Luh Nyoman Sri Kasihani P07134013031 Christian Naftali anni P0713401303! Ni Putu No"i Pus#ita Kusuma P07134013033 Ni $usti %yu Pra&nya '()i P07134013034 Ka&(* ')i S(#tini P0713401303+ I '()a $(&( %&itya Satria ', P, P0713401303- Luh Putu Su.iana Can&ra '()i P07134013037 %yu Nur /itriani P0713401303 $ustyari a&urani $iri P07134013032 S%N %N%LIS KESEH%%N POLIEKNIK KESEH%%N 'ENP%S% !01+ http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 1/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate PEMERIKSAAN GOLONGAN DARAH ABO DAN RHESUS Hari5tan66al S(lasa5!4 Mar(t !01+ %, u8uan a, u8uan Instru*sional mum Mahasis)a &a#at m(mahami .ara #(m(ri*saan 6olon6an &arah %9O &an h(sus, :, u8uan Instru*sional Khusus 1, Mahasis)a &a#at m(la*u*an .ara #(m(ri*saan 6olon6an &arah %9O &an h(sus, !, Mahasis)a &a#at m(n6int(r#r(tasi*an hasil #(m(ri*saan 6olon6an &arah %9O &an h(sus, 9, M(to&( M(to&( yan6 &i6una*an #a&a #ra*ti*um #(m(ri*saan 6olon6an &arah %9O &an h(sus a&alah m(to&( #lat(, C, Prinsi# %nti6(n ; %nti:o&y < %6lutinasi $olon6an &arah &ii&(ntifi*asi*an &(n6an m(lihat a6lutinasi yaitu #(n66um#alan s(l &arah m(rah a*i:at r(a*si antara %nti:o&y &alam s(rum atau #lasma &(n6an %nti6(n #a&a s(l &arah m(rah, ', 'asar (ori ranfusi &arah a&alah #ros(s m(nyalur*an &arah atau #ro&u* :(r:asis &arah &ari satu oran6 *( sist(m #(r(&aran &arah oran6 lainnya, ransfusi &arah :(rhu:un6an &(n6an *on&isi m(&is s(#(rti *(hilan6an &arah &alam 8umlah :(sar &is(:a:*an trauma= o#(rasi= syo*= &an ti&a* :(rfun6sinya or6an #(m:(ntu* s(l &arah m(rah, P(m(ri*saan #r(>transfusi :(rtu8uan untu* m(nilai *(.o.o*an &arah &onor &an &arah r(si#i(n &an m(n&(t(*si a&anya anti:o&i &alam &arah &onor mau#un #asi(n yan6 &a#atm(nim:ul*anr(a*si transfusi #a&a #asi(nyan6 &a#atm(nim:ul*anr(a*si transfusi #a&a #asi(n, http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 2/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate K(.o.o*an antara &arah &onor ?#(nyum:an6@ &an r(si#i(n ?#(n(rima@ san6at #(ntin6 &alam transfusi &arah, 'arah &onor &an r(si#i(n harus s(suai 6olon6annya :(r&asar*an sist(m %9O &an h(sus fa*tor, ?%nis !00@ Prinsip Dasar Penggolongan Darah a. /a*tor yan6 m(n(ntu*an 6olon6an &arah manusia :(ru#a anti6(n yan6 t(r&a#at #a&a #(rmu*aan luar s(l &arah m(rah &is(:ut Aglutinogen. . Aat anti t(rha&a# anti6(n t(rs(:ut &is(:ut Bat anti atau anti:o&i yan6 :ila :(r(a*si a*an m(n6han.ur*an anti6(n yan6 :(rsan6*utan &is(:ut Aglutinin &alam #lasma= suatu anti:o&i alamiah yan6 s(.ara otomatis t(r&a#at #a&a tu:uh manusia Darah 'arah a&alah .airan yan6 :(r)arna m(rah yan6 t(r&a#at &alam #(m:uluh &arah, Volum( &arah manusia 7 D &ari :(rat :a&an atau + lit(r untu* la*ila*i &an 4=+ lit(r untu* #(r(m#uan, 'arah m(m#unyai fun6si antara lain m(n6an6*ut o*si6(n &ari #aru>#aru *( s(luruh tu:uh= m(n6an6*ut *ar:on&io*sio&a &ari 8arin6an tu:uh *( #aru>#aru= m(n6an6*ut sari> sari ma*anan *( s(luruh tu:uh= m(n6an6*ut sisa>sisa ma*anan &ari s(luruh 8arin6an tu:uh *( alat>alat (*s*r(si= m(n6an6*ut hormon &ari *(l(n8ar (n&o*rin *( :a6ian tu:uh t(rt(ntu= m(n6an6*ut air untu* &i(&ar*an *( s(luruh tu:uh Golongan Darah $olon6an &arah a&alah #(n6*lasifi*asian &arah &ari suatu in&i"i&u :(r&asar*an a&a atau ti&a* a&anya Bat anti6(n )arisan #a&a #(rmu*aan m(m:ran s(l &arah m(rah, Hal ini &is(:a:*an *ar(na a&anya #(r:(&aan 8(nis *ar:ohi&rat &an #rot(in #a&a #(rmu*aan m(m:ran s(l &arah m(rah t(rs(:ut, Sist(m #(n66olon6an &arah :(sar yan6 &i*(nal a&alah sist(m %9O ?6olon6an &arah %= 9= %9= &an O@ s(rta sist(m #(n66olon6an &arah h(sus ?h; &an h>@, $olon6an &arah manusia &it(ntu*an :(r&asar*an 8(nis anti6(n &an anti:o&i yan6 t(r*an&un6 &alam &arahnya= s(:a6ai :(ri*ut • In&i"i&u &(n6an 6olon6an &arah % m(mili*i s(l &arah m(rah &(n6an anti6(n % &i #(rmu*aan m(m:ran s(lnya &an m(n6hasil*an anti:o&i t(rha&a# anti6(n 9 &alam s(rum &arahnya, http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 3/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate • In&i"i&u &(n6an 6olon6an &arah 9 m(mili*i anti6(n 9 #a&a #(rmu*aan s(l &arah m(rahnya &an m(n6hasil*an anti:o&i t(rha&a# anti6(n % &alam s(rum &arahnya • In&i"i&u &(n6an 6olon6an &arah %9 m(mili*i s(l &arah m(rah &(n6an anti6(n % &an 9 s(rta ti&a* m(n6hasil*an anti:o&i t(rha&a# anti6(n % mau#un 9 • In&i"i&u &(n6an 6olon6an &arah O m(mili*i s(l &arah tan#a anti6(n= ta#i m(m#ro&u*si anti:o&i t(rha&a# anti6(n % &an 9, Rhesus h(sus a&alah sist(m #(n66olon6an &arah :(r&asar*an a&a atau ti&a*nya anti6(n ' &i #(rmu*aan s(l &arah m(rah= nama lainnya a&alah faktor Rhesus atau faktor Rh, Nama ini &i#(rol(h &ari mony(t 8(nis h(sus yan6 &i*(tahui m(mili*i fa*tor ini #a&a tahun 1240 ol(h Karl Lan&st(in(r, S(s(oran6 yan6 ti&a* m(mili*i fa*tor h &i #(rmu*aan s(l &arah m(rahnya m(mili*i 6olon6an &arah h> ?h(sus N(6atif@, M(r(*a yan6 m(mili*i fa*tor h #a&a #(rmu*aan s(l &arah m(rahnya &is(:ut m(mili*i 6olon6an &arah h; ?h(sus Positif@, (nis #(n66olon6an ini s(rin6*ali &i6a:un6*an &(n6an #(n66olon6an %9O &(n6an m(nam:ah*an F;G :a6i #(mili* fa*tor rh(sus atau F>F :a6i yan6 ti&a* m(mili*i fa*tor rh(sus &alam &arahnya= s(hin66a *ita m(n6(nal 6olon6an &arah % ; atau %>= 9; atau 9>= %9; atau %9>= &an O; atau O, E, %lat= 9ahan= (a6(n a, %lat 1, 9loo&6rou#in6 Plat( !, Pi#(t Past(ur 3, Pul#(n &an la:(l :, 9ahan 1, S(rum &onor !, Sus#(nsi (ritrosit +D &onor 3, Sus#(nsi (ritrosit 10D &onor 4, Sus#(nsi (ritrosit 40D &onor ., (a6(n http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 4/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate 1, %nti>% !, %nti>9 3, S(l (ri % 10D 4, S(l (ri 9 10D +, S(l (ri O 10D -, %nti>' 7, 9o"in %l:umin !!D /, Cara K(r8a 1, 'i:uat sus#(nsi (ritrosit +D= 10D &an 40D s(rta &isia#*an s(rumnya, !, 'i:(ri la:(l #a&a masin6>masin6 sumur &alam :loo&6rou#in6 #lat(, 3, 'it(t(s*an #a&a sumur &alam :loo&6rou#in6 #lat( > Sumur 1 ! t(t(s %nti>% > Sumur ! ! t(t(s %nti>9 > Sumur 3 1 t(t(s s(l (ri % 10D > Sumur 4 1 t(t(s s(l (ri 9 10D > Sumur + 1 t(t(s s(l (ri O 10D > Sumur 7 ! t(t(s %nti>' > Sumur ! t(t(s 9o"in %l:umin !!D 4, 'it(t(s*an 1 t(t(s sus#(nsi (ritrosit 10D #a&a sumur 1=! &an -, +, 'it(t(s*an 1 t(t(s sus#(nsi (ritrosit 40D #a&a sumur 7 &an , -, 'it(t(s*an masin6>masin6 ! t(t(s s(rum #a&a sumur 3=4=+= &an -, 7, 'i6oyan6*an :loo&6rou#in6 #lat( *( &(#an &an *( :(la*an6 hin66a t(r.am#ur &an &iamati a#a*ah t(r:(ntu* a6lutinasi, http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 5/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate $, Hasil P(n6amatan a, S*(ma K(r8a $am:ar K(t(ran6an Sumur 1 ! t(t(s %nti>% ; 1 t(t(s Sus#(nsi 10D Sumur ! ! t(t(s %nti>9 ; 1 t(t(s Sus#(nsi 10D Sumur 3 1 t(t(s (ri % 10D ; ! t(t(s s(rum Sumur 4 1 t(t(s (ri 9 10D ; ! t(t(s s(rum Sumur + 1 t(t(s (ri O 10D ; ! t(t(s s(rum Sumur - 1 t(t(s Sus#(nsi 10D ; ! t(t(s s(rum Sumur 7 ! t(t(s %nti>' ; 1 t(t(s Sus#(nsi 40D Sumur ! t(t(s 9o"in %l:umin !!D ; 1 t(t(s Sus#(nsi 40D :, Hasil Pra*ti*um $am:ar K(t(ran6an Golongan Darah ! B" Sumur 1 N(6atif ?>@ Sumur ! %6lutinasi ?;@ Sumur 3 %6lutinasi ?;@ Sumur 4 N(6atif ?>@ Sumur + N(6atif ?>@ Sumur - N(6atif ?>@ Sumur 7 %6lutinasi ?;@ Sumur N(6atif ?>@ ., 'ata $olon6an 'arah %= 9= %9 &an O No 1, $am:ar K(t(ran6an $olon6an 'arah %; %6lutinasi #a&a antis(ra %= s(l (ri 9 &an %nti ' http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 6/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate !, $olon6an 'arah 9; %6lutinasi #a&a antis(ra 9= s(l (ri % &an %nti ' 3, $olon6an 'arah %9; %6lutinasi #a&a antis(ra %= antis(ra 9= &an %nti ' 4, $olon6an 'arah O; %6lutinasi #a&a s(l (ri %= s(l (ri 9= &an %nti ' H, P(m:ahasan 'arah a&alah .airan yan6 t(r&a#at #a&a s(mua ma*hlu* hi&u# ?*(.uali tum:uhan@ tin6*at tin66i yan6 :(rfun6si m(n6irim*an Bat>Bat &an o*si6(n yan6 &i:utuh*an ol(h 8arin6an tu:uh= m(n6an6*ut :ahan>:ahan, $olon6an &arah a&alah #(n6*lasifi*asian &arah &ari suatu in&i"i&u :(r&asar*an a&a atau ti&a* a&anya Bat anti6(n )arisan #a&a #(rmu*aan m(m:ran s(l &arah m(rah, Hal ini &is(:a:*an *ar(na a&anya #(r:(&aan 8(nis *ar:ohi&rat &an #rot(in #a&a #(rmu*aan m(m:ran s(l &arah m(rah t(rs(:ut, M(nurut sist(m %9O= 6olon6an &arah manusia &i:(&a*an m(n8a&i (m#at= yaitu s(:a6ai :(ri*ut % %#a:ila &i &alam s(l &arah s(s(oran6 m(n6an&un6 a6lutino6(n % & an s(rumnya m(n6an&un6 a6lutinin s(hin66a &a#at &irumus*an ?%= @, 9 %#a:ila &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n 9= s(&an6*an &alam s(rumnya t(r&a#at a6lutinin Js(hin66a &irumus*an ?9= J @ %9 %#a:ila &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n % &an 9= s(&an6*an &i &alam s(rumnya ti&a* m(n6an&un6 a6lutinin= s(hin66a &a#at &irumus*an ?%9=@ http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 7/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate O %#a:ila &i &alam s(l &arah s(s(oran6 ti&a* t(r&a#at a6lutino6(n s(&an6*an &alam s(rumnya m(n6an&un6 a6lutinin J &an s(hin66a &a#at &irumus*an ?>= J= @, K(mu&ian 6olon6an &arah &i:(&a*an m(n8a&i &ua= yaitu s(:a6ai :(ri*ut 1, $olon6an &arah h;= 8i*a &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n rh(sus, !, $olon6an &arah h= 8i*a &i &alam s(l &arah s(s(oran6 ti&a* t(r&a#at a6lutino6(n rh(sus, Pa&a Pra*ti*um *ali ini *ita m(la*u*an #ra*ti*um t(ntan6 #(n(ntuan 6olon6an &arah &an rh(sus, Prinsi# &ari #(n66olon6an &arah ini a&alah $olon6an &arah &iin&(ntifi*asi*an &(n6an m(lihat a6lutinasi yaitu #(n66um#alan s(l &arah m(rah a*i:at r(a*si antara anti:o&i &alam s(rum5#lasma &(n6an anti6(n #a&a s(l &arah m(rah Pa&a #ra*ti*um *ali ini &i6una*an sam#(l s(l &arah m(rah &(n6an sus#(nsi 10D= 40D &an s(rum, 'i6una*an :loo&6rou#in6 #lat( untu* m(n(ntu*an 6olon6an &arah ini, %&a#un &alam #ra*ti*um ini &ila*u*an= s(:a6ai :(ri*ut 1, Sumur #(rtama :(risi ! t(t(s %nti>% &an 1 t(t(s sus#(nsi 10D !, Sumur *(&ua :(risi ! t(t(s %nti>9 &an 1 t(t(s sus#(nsi 10D 3, Sumur *(ti6a :(risi 1 t(t(s Eri % &an ! t(t(s s(rum 4, Sumur *((m#at :(risi 1 t(t(s Eri 9 &an ! t(t(s s(rum +, 'umur *(lima :(risi 1 t(t(s Eri O &an ! t(t(s s(rum -, Sumur *((nam a&alah auto .ontrol &imana isinya a&alah 1 t(t(s sus#(nsi 10D &an ! t(t(s s(rum, 7, Sumur *(tu8uh :(risi ! t(t(s %nti>' &an 1 t(t(s sus#(nsi s(l 40D , Sumur *(&(la#an :(risi ! t(t(s 9o"in al:umin &an 1 t(t(s sus#(nsi s(l 40D S(t(lah s(mua sumur yan6 &i6una*an t(risi :loo&6rou#in6 #lat( &i6oyan6*an sam:il &iamati a#a*ah t(:(ntu* a6*utinasi atau ti&a*, 'ari hasil yan6 &i#(rol(h &a#at &i:an&in6*an &(n6a ta:(l &i:a)ah ini ?&imana ; ?a&a a6lutinasi@ &an ?ti&a* a&a a6lutinasi@@, http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 8/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate Forward grouping Gol. Reverse grouping Anti A Anti B Anti A#B Sel A Sel B Sel O A " $ " $ " $ B $ " " " $ $ AB " " " $ $ $ O $ $ $ " " $ Oh $ $ $ " " " S(&an6*an untu* sumur yan6 :(risi %nti>' m(ru#a*an sumur yan6 &i6una*an untu* #(n(ntuan h(sus= 8i*a #a&a sumur t(rs(:ut t(r&a#at a6lutinasi :(rarti h(sus &arah t(rs(:ut a&alah #ositif s(&an6*an 8i*a ti&a* a&a :(rarti h(sus t(rs(:ut a&alah n(6atif, 'ari #ra*ti*um yan6 *ita la*u*an &i#(rol(h &ata %nti %nti Eri % Eri 9 Eri O %uto %nti 9o"in % > 9 ; ; > > .ontrol > ' ; al:umin > a&i= hasil yan6 &i&a#at*an #a&a #ra*ti*um *ali ini a&alah &arah yan6 :(r6olon6an &arah 9 &an m(mili*i h(sus #ositif, 'alam transfusi &arah= *(.o.o*an 6olon6an &arah mutla* &i#(rhati*an :ila a*an m(la*u*an transfusi &arah, 'alam m(la*u*an transfusi &arah s(:isa mun6*in &ila*u*an #a&a 6olon6an &arah yan6sama= :ila t(r#a*sa ti&a* t(rs(&ia 6olon6an &arah yan6 sama= ma*a &a#at &i6una*an &(n6an 6olon6an &arah yan6 :(r:(&a asal*an masih &alam tin6*at *(.o.o*an, Kar(na :ila ti&a* m(m#(rhati*an tin6*at *(.o.o*an antara 6olon6an &arah yan6 :(r:(&a ma*a a*an :(ra*i:at fatal #a&a #asi(n, http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 9/11 7/18/2019 I, Laporan Transfusi Golongan Darah Metode Bioplate K(sim#ulan 1, P(m(ri*saan 6olon6an &arah %9O &an h(sus &(n6an m(to&( #lat( &ila*u*an m(n66una*an :loo&6rou#in6 #lat( &an :(:(ra#a r(a6(n yaitu %nti>%= %nti>9= S(l (ri % 10D= S(l (ri 9 10D= S(l (ri O 10D= %nti>' &an 9o"in %l:umin !!D= s(rta &i6una*an sam#(l :(ru#a s(rum= sus#(nsi (ritrosit +D= sus#(nsi (ritrosit 10D= &an sus#(nsi (ritrosit 40D, !, P(m(ri*saan 6olon6an &arah %9O &an h(sus t(rha&a# sam#(l &onor= &i&a#at*an hasil 6olon6an &arah &onor a&alah 9;= &imana t(r:(ntu* a6lutinasi #a&a %nti>9= S(l (ri % &an %nti>' #a&a #(m(ri*saan= yan6 m(nun8u**an &i &alam s(l &arah &onor t(r&a#at a6lutino6(n 9= s(&an6*an &alam s(rumnya t(r&a#at a6lutinin J ?9= J @ s(rta &i &alam s(l &arah &onor t(r&a#at a6lutino6(n rh(sus, , 'aftar Pusta*a %&min, !013, Pemeriksaan Sederhana Golongan Darah dan Rhesus, htt#55))),rh(susn(6atif,.om5arti.l(&(tail,#h#i&1+7 ?&i*as(s !+ Mar(t !01+@ Satria= Imam, !013, Laporan Biologi Pemeriksaan Golongan Darah, htt#55imam6(ry4+,:lo6s#ot,.om5!0135115la#oran>:iolo6i>#(m(ri*saan> 6olon6an,html ?&i*as(s !+ Mar(t !01+@ Ina)ati, !013, Uji Golongan Darah, htt#55ina>s.i(ntist,:lo6s#ot,.om5!0135115u8i> 6olon6an>&arah,html ?&i*as(s !+ Mar(t !01+@ %riot(8o= 9ima, !014, Sistem Golongan Darah, htt#s55:imaariot(8o,)or&#r(ss,.om5!01450-50-5sist(m>6olon6an>&arah5 ?&i*as(s !+ Mar(t !01+@ '(n#asar= 31 Mar(t !01+ Pra*ti*an a5n K(lom#o* IV Ni Putu No"i Pus#ita Kusuma http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 10/11 7/18/2019 Laporan Transfusi Golongan Darah Metode Bioplate LEMBAR PENGESAHAN M(n6(tahui= P(m:im:in6 I P(m:im:in6 II 'r, Ni Ka&(* Mulyantari= S#,PK Ka&(* %rya&i harta)i6una= %,M&,%K P(m:im:in6 III P(m:im:in6 IV I $(&( Putu Su&ana Ni Ma&( 'armaasih P(m:im:in6 V $usti %yu N6urah ar&ani http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate 11/11