dokumen.tips laporan-transfusi-golongan-darah-metode-bioplate

7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
LAPORAN
PEMERIKSAAN GOLONGAN DARAH ABO
DAN RHESUS
OLEH
KELOMPOK IV
Ni Luh Nyoman Sri Kasihani
P07134013031
Christian Naftali anni
P0713401303!
Ni Putu No"i Pus#ita Kusuma
P07134013033
Ni $usti %yu Pra&nya '()i
P07134013034
Ka&(* ')i S(#tini
P0713401303+
I '()a $(&( %&itya Satria ', P,
P0713401303-
Luh Putu Su.iana Can&ra '()i
P07134013037
%yu Nur /itriani
P0713401303
$ustyari a&urani $iri
P07134013032
S%N %N%LIS KESEH%%N
POLIEKNIK KESEH%%N 'ENP%S%
!01+
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1/11
7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
PEMERIKSAAN GOLONGAN DARAH ABO
DAN RHESUS
Hari5tan66al
 S(lasa5!4 Mar(t !01+
%, u8uan
a, u8uan Instru*sional mum
Mahasis)a &a#at m(mahami .ara #(m(ri*saan 6olon6an &arah %9O &an h(sus,
:, u8uan Instru*sional Khusus
1, Mahasis)a &a#at m(la*u*an .ara #(m(ri*saan 6olon6an &arah %9O &an h(sus,
!, Mahasis)a &a#at m(n6int(r#r(tasi*an hasil #(m(ri*saan 6olon6an &arah %9O
&an h(sus,
9, M(to&(
M(to&( yan6 &i6una*an #a&a #ra*ti*um #(m(ri*saan 6olon6an &arah %9O &an h(sus
a&alah m(to&( #lat(,
C, Prinsi#
%nti6(n ; %nti:o&y < %6lutinasi
$olon6an &arah &ii&(ntifi*asi*an &(n6an m(lihat a6lutinasi yaitu #(n66um#alan s(l
&arah m(rah a*i:at r(a*si antara %nti:o&y &alam s(rum atau #lasma &(n6an %nti6(n #a&a
s(l &arah m(rah,
', 'asar (ori
ranfusi &arah a&alah #ros(s m(nyalur*an &arah atau #ro&u* :(r:asis &arah &ari
satu oran6 *( sist(m #(r(&aran &arah oran6 lainnya, ransfusi &arah :(rhu:un6an &(n6an
*on&isi m(&is s(#(rti *(hilan6an &arah &alam 8umlah :(sar &is(:a:*an trauma= o#(rasi=
syo*= &an ti&a* :(rfun6sinya or6an #(m:(ntu* s(l &arah m(rah,
P(m(ri*saan #r(>transfusi :(rtu8uan untu* m(nilai *(.o.o*an &arah &onor &an
&arah r(si#i(n &an m(n&(t(*si a&anya anti:o&i &alam &arah &onor mau#un #asi(n yan6
&a#atm(nim:ul*anr(a*si transfusi #a&a #asi(nyan6 &a#atm(nim:ul*anr(a*si transfusi
#a&a #asi(n,
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2/11
7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
K(.o.o*an antara &arah &onor ?#(nyum:an6@ &an r(si#i(n ?#(n(rima@ san6at
#(ntin6 &alam transfusi &arah, 'arah &onor &an r(si#i(n harus s(suai 6olon6annya
:(r&asar*an sist(m %9O &an h(sus fa*tor, ?%nis  !00@
Prinsip Dasar Penggolongan Darah
a. /a*tor yan6 m(n(ntu*an 6olon6an &arah manusia :(ru#a anti6(n yan6 t(r&a#at #a&a
#(rmu*aan luar s(l &arah m(rah &is(:ut Aglutinogen.
. Aat anti t(rha&a# anti6(n t(rs(:ut &is(:ut Bat anti atau anti:o&i yan6 :ila :(r(a*si
a*an m(n6han.ur*an anti6(n yan6 :(rsan6*utan &is(:ut Aglutinin &alam #lasma=
suatu anti:o&i alamiah yan6 s(.ara otomatis t(r&a#at #a&a tu:uh manusia
Darah
'arah a&alah .airan yan6 :(r)arna m(rah yan6 t(r&a#at &alam #(m:uluh &arah,
Volum( &arah manusia  7 D &ari :(rat :a&an atau  + lit(r untu* la*ila*i &an 4=+ lit(r
untu* #(r(m#uan,
'arah m(m#unyai fun6si antara lain m(n6an6*ut o*si6(n &ari #aru>#aru *( s(luruh
tu:uh= m(n6an6*ut *ar:on&io*sio&a &ari 8arin6an tu:uh *( #aru>#aru= m(n6an6*ut sari>
sari ma*anan *( s(luruh tu:uh= m(n6an6*ut sisa>sisa ma*anan &ari s(luruh 8arin6an
tu:uh *( alat>alat (*s*r(si= m(n6an6*ut hormon &ari *(l(n8ar (n&o*rin *( :a6ian tu:uh
t(rt(ntu= m(n6an6*ut air untu* &i(&ar*an *( s(luruh tu:uh
Golongan Darah
$olon6an &arah a&alah #(n6*lasifi*asian &arah &ari suatu in&i"i&u :(r&asar*an a&a
atau ti&a* a&anya Bat anti6(n )arisan #a&a #(rmu*aan m(m:ran s(l &arah m(rah, Hal ini
&is(:a:*an *ar(na a&anya #(r:(&aan 8(nis *ar:ohi&rat &an #rot(in #a&a #(rmu*aan
m(m:ran s(l &arah m(rah t(rs(:ut,
Sist(m #(n66olon6an &arah :(sar yan6 &i*(nal a&alah sist(m %9O ?6olon6an &arah
%= 9= %9= &an O@ s(rta sist(m #(n66olon6an &arah h(sus ?h; &an h>@,
$olon6an &arah manusia &it(ntu*an :(r&asar*an 8(nis anti6(n &an anti:o&i yan6
t(r*an&un6 &alam &arahnya= s(:a6ai :(ri*ut
•
In&i"i&u &(n6an 6olon6an &arah % m(mili*i s(l &arah m(rah &(n6an anti6(n % &i
#(rmu*aan m(m:ran s(lnya &an m(n6hasil*an anti:o&i t(rha&a# anti6(n 9 &alam
s(rum &arahnya,
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3/11
7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
•
In&i"i&u &(n6an 6olon6an &arah 9 m(mili*i anti6(n 9 #a&a #(rmu*aan s(l &arah
m(rahnya &an m(n6hasil*an anti:o&i t(rha&a# anti6(n % &alam s(rum &arahnya
•
In&i"i&u &(n6an 6olon6an &arah %9 m(mili*i s(l &arah m(rah &(n6an anti6(n % &an
9 s(rta ti&a* m(n6hasil*an anti:o&i t(rha&a# anti6(n % mau#un 9
•
In&i"i&u &(n6an 6olon6an &arah O m(mili*i s(l &arah tan#a anti6(n= ta#i
m(m#ro&u*si anti:o&i t(rha&a# anti6(n % &an 9,
Rhesus
h(sus a&alah sist(m #(n66olon6an &arah :(r&asar*an a&a atau ti&a*nya anti6(n '
&i #(rmu*aan s(l &arah m(rah= nama lainnya a&alah faktor Rhesus atau faktor Rh, Nama
ini &i#(rol(h &ari mony(t 8(nis h(sus yan6 &i*(tahui m(mili*i fa*tor ini #a&a tahun
1240 ol(h Karl Lan&st(in(r,
S(s(oran6 yan6 ti&a* m(mili*i fa*tor h &i #(rmu*aan s(l &arah m(rahnya
m(mili*i 6olon6an &arah h> ?h(sus N(6atif@, M(r(*a yan6 m(mili*i fa*tor h #a&a
#(rmu*aan s(l &arah m(rahnya &is(:ut m(mili*i 6olon6an &arah h; ?h(sus Positif@,
(nis #(n66olon6an ini s(rin6*ali &i6a:un6*an &(n6an #(n66olon6an %9O &(n6an
m(nam:ah*an F;G :a6i #(mili* fa*tor rh(sus atau F>F :a6i yan6 ti&a* m(mili*i fa*tor
rh(sus &alam &arahnya= s(hin66a *ita m(n6(nal 6olon6an &arah % ; atau %>= 9; atau 9>=
%9; atau %9>= &an O; atau O,
E, %lat= 9ahan= (a6(n
a, %lat
1, 9loo&6rou#in6 Plat(
!, Pi#(t Past(ur
3, Pul#(n &an la:(l
:, 9ahan
1, S(rum &onor
!, Sus#(nsi (ritrosit +D &onor
3, Sus#(nsi (ritrosit 10D &onor
4, Sus#(nsi (ritrosit 40D &onor
., (a6(n
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Laporan Transfusi Golongan Darah Metode Bioplate
1, %nti>%
!, %nti>9
3, S(l (ri % 10D
4, S(l (ri 9 10D
+, S(l (ri O 10D
-, %nti>'
7, 9o"in %l:umin !!D
/, Cara K(r8a
1, 'i:uat sus#(nsi (ritrosit +D= 10D &an 40D s(rta &isia#*an s(rumnya,
!, 'i:(ri la:(l #a&a masin6>masin6 sumur &alam :loo&6rou#in6 #lat(,
3, 'it(t(s*an #a&a sumur &alam :loo&6rou#in6 #lat( 
>
Sumur 1  ! t(t(s %nti>%
>
Sumur !  ! t(t(s %nti>9
>
Sumur 3  1 t(t(s s(l (ri % 10D
>
Sumur 4  1 t(t(s s(l (ri 9 10D
>
Sumur +  1 t(t(s s(l (ri O 10D
>
Sumur 7  ! t(t(s %nti>'
>
Sumur   ! t(t(s 9o"in %l:umin !!D
4, 'it(t(s*an 1 t(t(s sus#(nsi (ritrosit 10D #a&a sumur 1=! &an -,
+, 'it(t(s*an 1 t(t(s sus#(nsi (ritrosit 40D #a&a sumur 7 &an ,
-, 'it(t(s*an masin6>masin6 ! t(t(s s(rum #a&a sumur 3=4=+= &an -,
7, 'i6oyan6*an :loo&6rou#in6 #lat( *( &(#an &an *( :(la*an6 hin66a t(r.am#ur &an
&iamati a#a*ah t(r:(ntu* a6lutinasi,
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Laporan Transfusi Golongan Darah Metode Bioplate
$, Hasil P(n6amatan
a, S*(ma K(r8a
$am:ar
K(t(ran6an
Sumur 1  ! t(t(s %nti>% ; 1 t(t(s Sus#(nsi 10D
Sumur !  ! t(t(s %nti>9 ; 1 t(t(s Sus#(nsi 10D
Sumur 3  1 t(t(s (ri % 10D ; ! t(t(s s(rum
Sumur 4  1 t(t(s (ri 9 10D ; ! t(t(s s(rum
Sumur +  1 t(t(s (ri O 10D ; ! t(t(s s(rum
Sumur -  1 t(t(s Sus#(nsi 10D ; ! t(t(s s(rum
Sumur 7  ! t(t(s %nti>' ; 1 t(t(s Sus#(nsi 40D
Sumur   ! t(t(s 9o"in %l:umin !!D ; 1 t(t(s
Sus#(nsi 40D
:, Hasil Pra*ti*um
$am:ar
K(t(ran6an
Golongan Darah ! B"
Sumur 1  N(6atif ?>@
Sumur !  %6lutinasi ?;@
Sumur 3  %6lutinasi ?;@
Sumur 4  N(6atif ?>@
Sumur +  N(6atif ?>@
Sumur -  N(6atif ?>@
Sumur 7  %6lutinasi ?;@
Sumur   N(6atif ?>@
., 'ata $olon6an 'arah %= 9= %9 &an O
No
1,
$am:ar
K(t(ran6an
$olon6an 'arah  %;
%6lutinasi #a&a antis(ra %= s(l (ri 9 &an %nti '
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Laporan Transfusi Golongan Darah Metode Bioplate
!,
$olon6an 'arah  9;
%6lutinasi #a&a antis(ra 9= s(l (ri % &an %nti '
3,
$olon6an 'arah  %9;
%6lutinasi #a&a antis(ra %= antis(ra 9= &an %nti '
4,
$olon6an 'arah  O;
%6lutinasi #a&a s(l (ri %= s(l (ri 9= &an %nti '
H, P(m:ahasan
'arah a&alah .airan yan6 t(r&a#at #a&a s(mua ma*hlu* hi&u# ?*(.uali tum:uhan@
tin6*at tin66i yan6 :(rfun6si m(n6irim*an Bat>Bat &an o*si6(n yan6 &i:utuh*an ol(h
8arin6an tu:uh= m(n6an6*ut :ahan>:ahan, $olon6an &arah a&alah #(n6*lasifi*asian &arah
&ari suatu in&i"i&u :(r&asar*an a&a atau ti&a* a&anya Bat anti6(n )arisan #a&a
#(rmu*aan m(m:ran s(l &arah m(rah, Hal ini &is(:a:*an *ar(na a&anya #(r:(&aan 8(nis
*ar:ohi&rat &an #rot(in #a&a #(rmu*aan m(m:ran s(l &arah m(rah t(rs(:ut, M(nurut
sist(m %9O= 6olon6an &arah manusia &i:(&a*an m(n8a&i (m#at= yaitu s(:a6ai :(ri*ut
%
 %#a:ila &i &alam s(l &arah s(s(oran6 m(n6an&un6 a6lutino6(n % & an
s(rumnya m(n6an&un6 a6lutinin  s(hin66a &a#at &irumus*an ?%=  @,
9
 %#a:ila &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n 9= s(&an6*an
&alam s(rumnya t(r&a#at a6lutinin Js(hin66a &irumus*an ?9= J @
%9
 %#a:ila &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n % &an 9= s(&an6*an
&i &alam s(rumnya ti&a* m(n6an&un6 a6lutinin= s(hin66a &a#at &irumus*an
?%9=@
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7/11
7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
O
 %#a:ila &i &alam s(l &arah s(s(oran6 ti&a* t(r&a#at a6lutino6(n s(&an6*an
&alam s(rumnya m(n6an&un6 a6lutinin J &an  s(hin66a &a#at &irumus*an ?>=
J=  @,
K(mu&ian 6olon6an &arah &i:(&a*an m(n8a&i &ua= yaitu s(:a6ai :(ri*ut
1, $olon6an &arah h;= 8i*a &i &alam s(l &arah s(s(oran6 t(r&a#at a6lutino6(n rh(sus,
!, $olon6an &arah h= 8i*a &i &alam s(l &arah s(s(oran6 ti&a* t(r&a#at a6lutino6(n
rh(sus,
Pa&a Pra*ti*um *ali ini *ita m(la*u*an #ra*ti*um t(ntan6 #(n(ntuan 6olon6an
&arah &an rh(sus, Prinsi# &ari #(n66olon6an &arah ini a&alah  $olon6an &arah
&iin&(ntifi*asi*an &(n6an m(lihat a6lutinasi yaitu #(n66um#alan s(l &arah m(rah a*i:at
r(a*si antara anti:o&i &alam s(rum5#lasma &(n6an anti6(n #a&a s(l &arah m(rah
Pa&a #ra*ti*um *ali ini &i6una*an sam#(l s(l &arah m(rah &(n6an sus#(nsi 10D=
40D &an s(rum, 'i6una*an :loo&6rou#in6 #lat( untu* m(n(ntu*an 6olon6an &arah ini,
%&a#un &alam #ra*ti*um ini &ila*u*an= s(:a6ai :(ri*ut 
1, Sumur #(rtama :(risi ! t(t(s %nti>% &an 1 t(t(s sus#(nsi 10D
!, Sumur *(&ua :(risi ! t(t(s %nti>9 &an 1 t(t(s sus#(nsi 10D
3, Sumur *(ti6a :(risi 1 t(t(s Eri % &an ! t(t(s s(rum
4, Sumur *((m#at :(risi 1 t(t(s Eri 9 &an ! t(t(s s(rum
+, 'umur *(lima :(risi 1 t(t(s Eri O &an ! t(t(s s(rum
-, Sumur *((nam a&alah auto .ontrol &imana isinya a&alah 1 t(t(s sus#(nsi 10D &an !
t(t(s s(rum,
7, Sumur *(tu8uh :(risi ! t(t(s %nti>' &an 1 t(t(s sus#(nsi s(l 40D
, Sumur *(&(la#an :(risi ! t(t(s 9o"in al:umin &an 1 t(t(s sus#(nsi s(l 40D
S(t(lah s(mua sumur yan6 &i6una*an t(risi :loo&6rou#in6 #lat( &i6oyan6*an
sam:il &iamati a#a*ah t(:(ntu* a6*utinasi atau ti&a*, 'ari hasil yan6 &i#(rol(h &a#at
&i:an&in6*an &(n6a ta:(l &i:a)ah ini ?&imana ; ?a&a a6lutinasi@ &an  ?ti&a* a&a
a6lutinasi@@,
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8/11
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Laporan Transfusi Golongan Darah Metode Bioplate
Forward grouping
Gol.
Reverse grouping
Anti A
Anti B
Anti A#B
Sel A
Sel B
Sel O
A
"
$
"
$
"
$
B
$
"
"
"
$
$
AB
"
"
"
$
$
$
O
$
$
$
"
"
$
Oh
$
$
$
"
"
"
S(&an6*an untu* sumur yan6 :(risi %nti>' m(ru#a*an sumur yan6 &i6una*an
untu* #(n(ntuan h(sus= 8i*a #a&a sumur t(rs(:ut t(r&a#at a6lutinasi :(rarti h(sus
&arah t(rs(:ut a&alah #ositif s(&an6*an 8i*a ti&a* a&a :(rarti h(sus t(rs(:ut a&alah
n(6atif,
'ari #ra*ti*um yan6 *ita la*u*an &i#(rol(h &ata 
%nti
%nti
Eri %
Eri 9
Eri O
%uto
%nti
9o"in
%
>
9
;
;
>
>
.ontrol
>
'
;
al:umin
>
a&i= hasil yan6 &i&a#at*an #a&a #ra*ti*um *ali ini a&alah &arah yan6 :(r6olon6an
&arah 9 &an m(mili*i h(sus #ositif,
'alam transfusi &arah= *(.o.o*an 6olon6an &arah mutla* &i#(rhati*an :ila a*an
m(la*u*an transfusi &arah, 'alam m(la*u*an transfusi &arah s(:isa mun6*in &ila*u*an
#a&a 6olon6an &arah yan6sama= :ila t(r#a*sa ti&a* t(rs(&ia 6olon6an &arah yan6 sama=
ma*a &a#at &i6una*an &(n6an 6olon6an &arah yan6 :(r:(&a asal*an masih &alam tin6*at
*(.o.o*an, Kar(na :ila ti&a* m(m#(rhati*an tin6*at *(.o.o*an antara 6olon6an &arah
yan6 :(r:(&a ma*a a*an :(ra*i:at fatal #a&a #asi(n,
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9/11
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I,
Laporan Transfusi Golongan Darah Metode Bioplate
K(sim#ulan
1, P(m(ri*saan 6olon6an &arah %9O &an h(sus &(n6an m(to&( #lat( &ila*u*an
m(n66una*an :loo&6rou#in6 #lat( &an :(:(ra#a r(a6(n yaitu %nti>%= %nti>9= S(l (ri
% 10D= S(l (ri 9 10D= S(l (ri O 10D= %nti>' &an 9o"in %l:umin !!D= s(rta
&i6una*an sam#(l :(ru#a s(rum= sus#(nsi (ritrosit +D= sus#(nsi (ritrosit 10D= &an
sus#(nsi (ritrosit 40D,
!, P(m(ri*saan 6olon6an &arah %9O &an h(sus t(rha&a# sam#(l &onor= &i&a#at*an
hasil 6olon6an &arah &onor a&alah 9;= &imana t(r:(ntu* a6lutinasi #a&a %nti>9= S(l
(ri % &an %nti>' #a&a #(m(ri*saan= yan6 m(nun8u**an &i &alam s(l &arah &onor
t(r&a#at a6lutino6(n 9= s(&an6*an &alam s(rumnya t(r&a#at a6lutinin J ?9= J @ s(rta
&i &alam s(l &arah &onor t(r&a#at a6lutino6(n rh(sus,
, 'aftar Pusta*a
%&min,
!013,
Pemeriksaan
Sederhana
Golongan
Darah
dan
Rhesus,
htt#55))),rh(susn(6atif,.om5arti.l(&(tail,#h#i&1+7 ?&i*as(s !+ Mar(t !01+@
Satria=
Imam,
!013,
Laporan
Biologi
Pemeriksaan
Golongan
Darah,
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http://slidepdf.com/reader/full/laporan-transfusi-golongan-darah-metode-bioplate
10/11
7/18/2019
Laporan Transfusi Golongan Darah Metode Bioplate
LEMBAR PENGESAHAN
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11/11