ABSTRACT RESISTANCE OF VANAME SHRIMP (Litopenaeus

ABSTRACT
RESISTANCE OF VANAME SHRIMP (Litopenaeus vannamei, Boone 1931)
ADDED WITH PROBIOTIC Bacillus sp. D2.2 AGAINST ARTIFICIAL
INFECTIONS OF Vibrio alginolyticus
By
EMA RAHMAWATI
The main problem in the cultivation of vaname (Litopenaeus vannamei) shrimp is
disease, one of disease agents is bacteria, for example genus Vibrio that caused
vibriosis infection. The safe effort of disease prevention is by using probiotics.
Bacillus sp. D2.2 which has antibacterial and biocontrol acvities can be used as
probiotics. Application of probiotics with Bacillus sp. D2.2 combined with white
sweet potato prebotics containing oligosaccharides could have a beneficial effect
on the host by stimulating microbial growth and selectively stimulating the
growth of certain bacteria in the digestive tract. The aim of this research was to
know the resilience of vaname shrimp that has been given probiotic 6% with 0%
(control) to infection bacterial of V. alginolyticus in vivo then histology test to
observe muscular damage caused by infection. The results showed that the use of
probiotics Bacillus sp. D2.2 6% in vivo and histopatology test were able to
provide better shrimp resistance against V. alginolyticus.
Keywords : probiotic, Bacillus sp. D2.2, Litopenaeus vannamei, V. alginolyticus.
ABSTRAK
KETAHANAN UDANG VANAME (Litopenaeus vannamei, Boone 1931)
YANG DIBERI PROBIOTIK Bacillus sp. D2.2 TERHADAP INFEKSI
Vibrio alginolyticus
Oleh
EMA RAHMAWATI
Permasalahan utama dalam budidaya udang vaname (Litopenaeus vannamei)
adalah penyakit, salah satu agen penyakit yaitu bakteri, misalnya genus Vibrio
penyebab infeksi vibriosis. Upaya penanggulangan penyakit yang aman yaitu
dengan probiotik. Bacillus sp. D2.2 yang memiliki akvitas antibakteri dan
biokontrol dapat dimanfaatkan sebagai probiotik. Aplikasi probiotik dengan
bakteri Bacillus sp. D2.2 dikombinasikan dengan prebotik ubi jalar putih yang
memiliki kandungan oligosakarida dapat memberikan efek menguntungkan bagi
inang dengan cara merangsang pertumbuhan mikroba dan secara selektif
merangsang pertumbuhan sejumlah bakteri tertentu di saluran pencernaan.
Penelitian ini dilakukan bertujuan untuk mengetahui ketahanan udang vaname
yang diberi probiotik 6% dan 0% (kontrol) terhadap infeksi bakteri V.
alginolyticus secara in vivo kemudian dilakukan uji histopatologi untuk melihat
kerusakan jaringan akibat infeksi. Hasil penelitan menunjukkan bahwa
penggunaan probiotik Bacillus sp. D2.2 6% secara in vivo dan uji histopatologi
mampu memberikan ketahanan udang lebih baik terhadap infeksi V. alginolyticus.
Kata kunci : probotik, Bacillus sp. D2.2, Litopenaeus vannamei, V. alginolyticus