daftar isi kata pengantar
advertisement
DAFTAR ISI KATA PENGANTAR ..................................................................................... iv DAFTAR ISI .................................................................................................... vii DAFTAR TABEL ............................................................................................ viii DAFTAR GAMBAR ....................................................................................... ix DAFTAR LAMPIRAN .................................................................................... x INTISARI......................................................................................................... xi ABSTRACT ....................................................................................................... xii BAB I. PENDAHULUAN ............................................................................... A. Latar Belakang ..................................................................................... B. Tujuan Penelitian ................................................................................. C. Manfaat Penelitian ........................................................................... … 1 1 3 3 BAB II. TINJAUAN PUSTAKA..................................................................... A. Daging .................................................................................................. B. Gen 12S ribosom RNA ........................................................................ C. Deoxyribonucleic Acid (DNA)............................................................. D. Isolasi DNA.......................................................................................... E. Pengukuran Kemurnian dan Konsentrasi DNA ................................... F. Polymerase Chain Reaction ................................................................. G. Elektroforesis Gel Agarose .................................................................. 4 4 6 6 9 11 11 14 BAB III. MATERI DAN METODE ................................................................ A. Materi ................................................................................................... B. Metode.................................................................................................. 1. Pembuatan bakso............................................................................ 2. Isolasi DNA.................................................................................... 3. Perhitungan konsentrasi dan kemurnian DNA............................... 4. Pembuatan agarose......................................................................... 5. Pengujian kualitatif DNA............................................................... 6. Amplifikasi PCR ............................................................................ 7. Elektroforesis hasil PCR ................................................................ 18 18 19 19 20 21 22 22 23 23 BAB IV. HASIL DAN PEMBAHASAN ........................................................ 25 BAB V. KESIMPULAN DAN SARAN.......................................................... A. Kesimpulan .......................................................................................... B. Saran..................................................................................................... 31 31 31 vi DAFTAR PUSTAKA ...................................................................................... 32 LAMPIRAN ..................................................................................................... 35 vii DAFTAR TABEL Halaman Tabel 1. Komposisi dan kandungan gizi daging............................................ 4 Tabel 2. Konsentrasi gel agarose dan ukuran molekul DNA ........................ 16 Tabel 3. Primer dan urutan basanya (5’-3’) ................................................... 19 Tabel 4. Konsentrasi bakso ............................................................................. 20 Tabel 5. Program analisa PCR ....................................................................... 23 Tabel 6. Hasil penghitungan kemurnian dan konsentrasi DNA .................... 26 viii DAFTAR GAMBAR Halaman Gambar 1. Basa nitrogen purin dan pirimidin ................................................ 8 Gambar 2. Ikatan basa purin dan pirimidin ...................................................... 8 Gambar 3. Struktur kovalen DNA ................................................................... 8 Gambar 4. Model struktur double helix menurut Watsons dan Crick ................ 9 Gambar 5. Skema representasi Polymerase Chain Reaction (PCR) .................. 14 Gambar 6. Metode elektroforesis gel agarose ................................................ 17 Gambar 7. Skema metode penelitian .............................................................. 24 Gambar 8. DNA hasil isolasi yang dielektroforesis pada 2% gel agarose ..... 25 Gambar 9. Hasil amplifikasi PCR bakso anjing yang dielektroforesis pada 2% gel agarose ..................................................................... ix 28 DAFTAR LAMPIRAN Halaman Lampiran 1. Penghitungan konsentrasi DNA .................................................. 35 Lampiran 2. Penghitungan kemurnian DNA ................................................... 37 Lampiran 3. Bahan Pembuatan TBE................................................................ 38 x