+,"" 1"1", a"e, .-2.m

AKTTVITAS PENANCI(APRADTI({L BEBAS EKSTRiK FENOL
DARI
BENAI,U CENCKEH
(F.ee Rd.ttal S.aiaEiag
A.nrii.s
of phdoric Entu.s Jtoh CIow
tur8ite)
Edisur$nbdrtrFnll'wchroln.
'lurusa Fmsi Fak
MrPA
uKrr Tomohoi
4h \\tt^hd"t.4Reh,.
4 .. t,n|n_1rtu
paeru 44aL,_.rpit,.h.q ahtu.an,.a7t-.,
:h"rr:tLl
h4
tlJrn Dat d.r\.
|::'.': ".'',!i!H'''
14. h'Bh a
":!
twda4ht \a ad Da.0tr,I.
"6
1C U-Fhe t
.4L
aJ ,
qd"u
4!J1'\qd l'qb 4 4^'".a'' 4''..d D!'i ;e
^.
..1 4) rrr.o P ]n^\",t t".tot ta
to",, ast.s\r Rtut
hAqe|ful@dtdy\qPn?LE\
Nsk6n dibiDA juni 20ffi dn!tujui
Jl (mpus uiscq azlu, Maado
&r,"! Fijiab,
las
'''
95 r t5,
r.oEhn kohm*^
1"1", a"e,
enair disuNeb@vahm
i-d,i r.-,
Fnyarir
.-2.m
*^*ir
^''r" k";k*.
r*di.*,k,r-,
jsiuns korci.rdmr: d 0.2).
Piodfi dfi v raba (edibrl da0 iotr.dien
d.g-- if*e*'i
p-y"kir
n4eddug$jml3hb.sfiiokj d siF*aremtik
(tum f.nora! fiavonoi4 &njn, ticis) d noi re0otik
(kardrroid, viramin c) ydg menjtiki subni,sj
-,ip-r..i,r,), s.N*" '.-r,r
s6k.,
.","-^"-;
m
rdapar darM sy]lm. buahb$hh. helbar dan
*'+,""
4i"_,a *a
ka,r., p.ii"dE
anhao$tcrcsis, orcoNrosh, iofimasi,
.oil
d,,-r*tu.r.h'^j".r, M."h*"d,ir.*ru;
h,
neieob!'t b.lbiciipflr"ri tp.n ddnrr hgsi,
hn'pouMdroh'{oldkrarlffK.ndpbbeBlu
didugabdkibenrd.igdfiiokiniaaiiokrid darm
b:itr Ftrbu,. sjalr, m b. q dr infdmdr rd*Ei
rJdhgq*nyJlslmDmha[on@qfmo]'}d4
rsu]i lk !lBa,iokdd.LdrenotiLeb,hi
bhu b.DtL rcnst,.h nM irr btu! dam:
blngJfu!4perc'minisddi,\motf,.md!i5
bebcnpa
b'eia'
u.ma bdltu
ceogk.h.
dd
*..1]'a's'd?3)sd""I'"*D*"i..l"dlkbh,
iuer m.nplnri sir {iir ndnturs,oml seDrn,
bdltru ebdtsa redurM uflrq!€t nd,titl, FAe r
B.oalu m.tup*d bMd ym! hsui surr
lail'fumflda'&ddmd(ffi'}adengs.mm4i@
shpdb.nilu.RlreiJ4gdlfudaadahrl
k*[c.ngr€hyedMb' dMEd.cJms!aEI't
ahis: ehnot, h.brcl stuinim |orida msi;n
larbomqElgelrolh.creGkLs5sklorjdArui|4
(Dm!'!dr a.mmyl A"-esti! 6mri,\
rdli4
r.r_
iEd
s dli Frcl' w)
direnil-r-riltriLhidmTir (DPPH) dlpsoleh
d{i
siema
cngJsms'aalddl$lpJfiMna}sasulNe,i
pilrb b.gian bu'lga, daui, baraig dan akar sd.lrb
diknng sinku sdpai kad4 rn
< J%
sqitrlrb cmm *iuk
barng. dsm dan buga) dinaukl
smpd
dinydak.tr sbagli ckuivaLs Nus^dii dara mgrg
dnoluifl i€nnrur ndxrl ( I I ). sebdyak 0,r dL lmh
qmpcldimsul$idaLmhbuqE!rl]ysnsdjb[8}B
tmh
n roiL lah dir.mhahki r nL lmbn vmilio 4%
(b^) &lam mdaior d divoneks s{ges sludrh
' pckar dli divoneks hsi.
diranbablcn r.5 nL Hcl
yang
dihrubsi slma
dip siqkai
benalu Englch
k d'rd
Gln,
s.ks
20
h.ih
pada suhu
dengm can yans
sda
tand
Hsilnya
xandunsa!
bii
lerkonddNasidjnyab(dslbagzimg^gcls!"I
jan. smper koudis disding mnsmdd
k.nd srins. sdmjubyr filhi diuapku ncdggu.akan
DPPSmtiurur(lr).DipcAbpk sbmy*2nLlmh
r.r rrifmir r-pikirbid€ir(DPP|r) 0: mM &lm ehdd4
c.ngkch. Kc.'npd eknEk kemudiin dirinbaos dan
dnjfpa p.da - r0 sbcrm diemard stuk pegujni.
uda rmh darirp
srama
14
!
Pdmtua ka.dscs iohr fcmr mmurut 0 0).
sebmy3l0,lnLdkMldaun*ngr.hdinAukk,nke
dirambahkri 2 mL eksta(
ke
dlm.dg}.h Berubannvs
tuning nsut ukkm
{em
cnsiesip€Drslapndik.r sehnjuhtap0dtrriiansir
€mkhn m.ijel !:0 msil. absorbansi diuk( Pa& r
J r 7
im dngrn speke,roromder Uv.vn Milon Roy 501
dihituns seb.gai
Akivit6 pohgkap ndjkrl bcbs
1- (absdrbansi smP.r/
*bnr r menit.
m;";i
Hasilnva
sddjtrhlq diranb.nk
2
nL hrubn
Absdbm, b-,m d.rar dLbcBda{r50
di.yrbrsn
$b.si
ms
'jm
grldl(g cbhk.
Prosdu pcidfua kmdungm tobl ,]avonoid
mdode (l r)
'nenssu,rr(an
dibbsbk ddss 5,7 mL rkuadB,0.3 mr- NaNolda l
dihbdnlr dengm 2 hL NaoH 1 M, k.mudid divonek
ds dicn pada r 5r0 nm. xa.duiss rdral flavoioid
t
R
g
3
€
vrDdrqar rod rdot n votroid&nhintrko
.68i
p.idnirn ini ditddks bddb kd ksdunsm tanol,
flavo.oid ds Idin ierkoiden5ai dal .ksn:r dmol
d i{mp bleim btolli msreh dhaiikd dllm hbel I
Ddi rmpat basis remin b$tu c.ngr.h ymg diuj i.
muaj6k rsuna m€mjliki lodusa r4ol navonoid
dan bii yas sisninkan. Hasil ini heisindikdikan
b'hea ekrFrt bciaro cengleh y3ie diuji krva arn
$nya{a fcnoLiL flrvonoid dh dil Dan dlb sm
kod'ihrif mnhjukkm barr*, kmdmg{ bbr fend.
kerirr ene'
be lcdadia
..rl
ad,.h202.J5]173.5J;r{,%64],09mg^&Ek$Id'
bagiro ar md'ghdLne junrah Lme bcer hmin
A[s4ekftrbssniilrl.bihbesadrjoidiekltlk
ekr6k
tutam
penctiri
ini, roet rlnot
ddM crsfur
d'urrdlisd nrd4llss F r lmc/
lJ ron k o JaL er5rBr dknnM srd^rri
ir.nrr! clnarlh
nds,rimi pe b$hw mh idj*an;bnu.
221.45:129'45|239.dI242.15ne/*!'EkchkFA
djbediiglcnd.ngebfusdi@d4b0ca8ewya
ck*rr artr bcBif
ddu
Tinssinya
pot& *hineea
kmFn.n
fendtik
ridlhnyr kaidqg rohl f.not
eksrml bmatu .ebgk.h b€dubmsh
t4suiq
deng4lrnYiEmtoks'd!'fd'rinlassruiicb4'm
.kb,ltb.lalucncki
.koz}b.mr!ca]ak.ndisbrnk ot.haddFsr€r.byawa kimia ydg d.pai beryctu s.ba!,i
Tabel
cl$hi( bd3rtr
dioknbn
I uftrr kdms tetnlvuoid dath
cd*.rrnmbju*Ia bshw.khk a*,
id rdingsj diira
d,f dd ara. Tobt i,moid.kft*
,l',bfug.d'ddabEcahcfu-dadsbhl33.0l:
1?3,55j t74.13 dm 6e,32 mcec H6 ini ftnujulre
bahle ada h!burgri )ans posir r,nbc krnduns
obh.rsdr
b6tug.
lhtonoidd.B4lJdughf.nohldmLmelb€o
ekf,( benaru c6s(eh Hat iii k{ou*jm dGebabkm
an,,\, fero'tb.uirdpolarfh nsea.Ldr3rrv r
krraxr p€d, lbot kb'rr Mj ar. Mfln' ( El tmr;
ellmedihmmhbexlfdedlanBsd6km
pencmab darm beb.Epa tapotr 0r,r6.t7) bahva
[email protected]€l@l
rin4i $peat rn nehrctde {bot 0o. srra.
itu,
bqi
:leJgumM&n'!ctrct.htlnuh'kbihfmpha
rTjn n pska; bilian yan! bei,nseR
lvab mtur @ $par dm bcryma .dkrar stu scm
dmiln rd dzlm * le,jidi konplekp.tifenot@ hdir
prda
baiy*
tu
rnek
bij! tatir
huu
o.
rq.ask
kh k
r o, kr€r
4o4s bar q;bu 15 r
'a
'hme
l'li]ebihdd''rdn:n'p.@!ljpmddpd[s]lduFda
ldFq i.not*J.nrui dM turo\ (20r okh teia ,tu.
bi&pdd'Fnimbmel$9b9'd'ots'd{b'o]otr
hai!
n:d4 ndi(lt b€b{ DPPH
aktivib p.ndskrp (r.@e.!,rp) Edikt b.bd
dri ersbrl b@h &rhe d'etui dm@ FtutrD
6ditd Lldf.nir2.p dneit(Dppt{) s.uunjn l
DPPB biisany! digunakan s.bas.i subrd uniuk
nae.ld@i
eriollid4. tudikal Dpps rd:lah
ndik:l b.bas'kivi6
sobir d n idina srtu ckkbn a6u
hidms.i meojadi nol.kutyes sbjt (r1) penlujis
iktivnas p.nanshp rrdiklt bebs DppH s.caB
sp.kororomde dilahl,i dasu memtsi*a. .kd:l
E
m dismiru uft,r h.isuku.f.k,€Mer; ddj
.]Gft}mfokhdjralDPPH'^bsodssipd?Jl7m
n.n@ s.basai n,tsi ue nor*ur a ordde d6
b€aodi.ksrrr *basli doksid .
iliditunjukm Nh d.ism @bahm rma tui
abso,tasi l.bil'
Har
b'€is eklmr bflrru ctrenh memrir, kenanrii
gbagll p€mgk'p ndikal b.bs dl ab 50?. Dl
logrehnmiir'p.Lonb.s*b€J@g*:Dd'ld
$bagsini[i@ksbriDFkirogm*sftr.
r;rhin
dk prr,ihiirraLrqo.,n4r,ni$ammdrir
lEdi s,
&!
w'l
'|I'
(rdinc)(,,J
oan
Bdb kE br
d cnbil]Llhddm
dsl
mqsilrt
rr
d,-o;h !s bJ , elrr
bli.aFdJtmglJldgE!)d!tfi:1p00()'
tu{arbururdar emL' in'mqfiurrirrN'tr
*[lebihiingjdjbmdi'gkac\{flbabngdmd-i
i."ir" -"sk"r' tP.n.ot s*'-v" "kti"ie -tr*dik"l
g,Li"i.**i",r't^,t-u"r", r"r"'r *"
n-n i-" u,r* trs r.t..mt -r*" "^ou
-g!.d,tug.'- Ir
.;t"l,-'&*".'.""h'd
u"d-F; -."-.
,rta
i,.
dstrh!.tkRjspJoad!bliurbs,u(ng}.hdJ
*.u".-*ta'.'"*'"it t"m'-t-'*u"e"
;\Mi ds
"ii"g
uo'x.",l"nolq:'t"*alutoortolt'
spd,oe,l*u
u"ry-o a"t*
.""-nr'.
"rtt-rt^
-tr*";ao
dmbu4]dibeditrgkmdenlmBtsTd!bkofgdl
rMsr ro dr Ns , f Fda k( L00 ppm Ganbs I
'
t"" .L' -r,*
L,.i -, .r*it .,,.u"'
elroa-p.d"
*"-i"p.a,* *u*n,me,
'--rt
"ee
d" d-.r- v-s b-,"r d,g-"t-*k''d*;
$baslipetrghdbd E:tsioklidli lipida d:lm bd'd
Sympos
im s.ns
Le;c
,oo4
nmujukttr signi6to
beBlu..nekeh
t-d-B,"
v-s
"r- '..'r'r,
s"'- b'ei"".k,;"t k""-* b*'r" c*s'd
*.ai" u-e *"*:,n- "x**-*a*t*u*
hJb[simln*'!Nspasglrpadd'k,lb.bd
r-a-*"
" ;d--"
r.
d,,i
mu
i
Ah sican chem
J rB''. rmd.,?u
iurkEslriiro R
rr.
BuJda s.
14.
t^otP.A.tglq n
ress.
r,rsa
'
.$'a "r-,
oldzr v.200l.7 rsz. l:o,,
Tim LL. whn. PJ.
so'
icq
rrtcJor
lr
cd
ae,
tachenBnv17:969 911
rg94,rI' O/Cre, S,.7l:
kmc LK BiY_& Hu&sY, chn
77:307 31t
JA oilcJffi se
qonda
s, Norasawa H
17 sa*alibm H Y
z_Y 2m0
Ashidr
(
rr ftv PM ds Hdbon. JB. l93e ta'ro& i, t/4',
t,i,t'".r',!, n". rwax" t*d".x w*\
19'c[feK.T.woneTY,weic'tY'HdmgY.wYLin
ri'93./n.r-,sflNd"r3a!{6a'
b"*g d- d*"
lee3.oxitubmaMajtr
^nBBN,sl&eryMK
ado I bod(Ed5) D!, /dFroe xdc!&
ial
Faor'.rz'.3r:2
16. suY-L.,.
" ;d-*' rr*r
a**i.
507
D
B
[*sb'r
1l).,1,,til2,3a,/c,,rt'
EtredsotrH.rlth(vol
buu. bcMF! ddrh d"n mssu nemnr
Ellis
2r
qq3rr8.i.
R"i"'dN r H"-r.rd Ps tu*to!n
Fedchen 4n: t38T139L
Mad}rN n 2002 r /s?a F&/ C,lsa 50: 341
?2
Molyreux P
sonsklu*Tin
200a
Js.i z!+"r'
?:
13. Ms{,czocPriar RL 1997.r asn.Faad'ahd