ISOLASI GEN PENGKODE -AMILASE DARI BAKTERI LAUT Vibrio sp

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LAMPIRAN
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LAMPIRAN A
Elektroforegram hasil sekuensing Gen 16S rRNA bakteri laut galur lokal
SFNB3
A. Gen 16S rRNA bakteri laut galur lokal SFNB3 dengan primer Bact-F1
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B. Gen 16S rRNA bakteri laut galur lokal SFNB3 dengan primer UniB1
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LAMPIRAN B
Elektroforegram hasil sekuensing fragmen DNA sisipan pada
plasmid rekombinan (Vsp.7)
A. Fragmen DNA sisipan Vsp.7 dengan primer M13F-pUC
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B. Fragmen DNA sisipan Vsp.7 dengan primer M13R-pUC
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LAMPIRAN C
Analisis urutan asam amino pengkode α-amilase pada genus Vibrio dengan
program Conserved Domains dari NCBI
A. Domain yang terdapat pada α-amilase V. parahaemolyticus RIMD
B. Domain pada α-amilase Vibrio sp. Ex25
C. Domain pada α-amilase Vibrio harveyi HY01
D. Domain pada α-amilase Vibrio alginolyticus 12G01
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E. Domain pada α-amilase Vibrio splendidus 12B01
F. Domain pada α-amilase Vibrio cholerae MZO-3
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LAMPIRAN D
Analisis urutan asam amino pengkode α-amilase pada beberapa spesies
dengan program Conserved Domains dari NCBI
A. Domain pada α-amilase Arabidopsis thaliana
B. Domain pada α-amilase B. subtilis
C. Domain pada α-amilase pendegradasi pati mentah Streptomyces vezuelae