xvii INTISARI Streptococcus sanguinis merupakan salah satu koloni

INTISARI
Streptococcus sanguinis merupakan salah satu koloni bakteri pioneer pada
acquired pellicle permukaan gigi, yang mempunyai peran penting dalam proses
pembentukan dan pematangan plak gigi dengan menyediakan perlekatan bagi
bakteri lainnya. Minyak atsiri merupakan salah satu zat aktif daun sirih kuning,
yang berpotensi mampu menghambat perlekatan bakteri pioneer dalam rongga
mulut. Penelitian ini bertujuan untuk mengetahui pengaruh minyak atsiri dan air
rebusan daun sirih kuning terhadap persentase perlekatan bakteri Streptococcus
sanguinis.
Terdapat 5 kelompok perlakuan dalam penelitian ini, yaitu kelompok
minyak atsiri daun sirih kuning konsentrasi 10%, kelompok air rebusan 35
lembar daun sirih kuning, kelompok emulgator, kelompok kontrol positif
(chlorhexidine gluconate 0,12%), dan kelompok kontrol negatif (akuades steril).
Subjek penelitian ini adalah bakteri Streptococcus sanguinis yang diperoleh dari
isolasi plak supragingiva seorang sukarelawan. Suspensi bakteri sebanyak 0,1 l
ditambahkan ke dalam setiap tabung perlakuan dan diinkubasi selama 18 jam
pada suhu 37oC dengan kemiringan tabung reaksi 30o. Perlekatan bakteri pada
permukaan dinding tabung reaksi diukur dengan spektrofotometer 600 nm. Nilai
densitas optik dari hasil pengukuran dikonversikan ke dalam persentase
perlekatan bakteri.
Hasil uji ANAVA satu jalur menunjukkan adanya perbedaan signifikan
antara minyak atsiri dengan air rebusan daun sirih kuning (p<0,05). Kesimpulan
penelitian ini adalah terdapat pengaruh minyak atsiri dan air rebusan daun sirih
kuning terhadap persentase perlekatan bakteri Streptococcus sanguinis. Minyak
atsiri daun sirih kuning konsentrasi 10% dapat menurunkan persentase perlekatan
bakteri Streptococcus sanguinis lebih baik daripada air rebusan 35 lembar daun
sirih kuning.
Kata kunci: sirih kuning, minyak atsiri, air rebusan, adhesi, Streptococcus
sanguinis.
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ABSTRACT
Streptococcus sanguinis is one of the pioneer bacterial colonies on the
acquired pellicle of tooth surface, which has important role in the formation and
maturation of dental plaque by providing attachment other bacteria. Volatile oils
is the chief component of the yellow betle leaves, potentially able to inhibit the
attachment of bacteria pioneer in the oral cavity. The aim of this study was to
know the effect of volatile oils and boiled water of yellow betle leaves against the
percentage adherence of the bacteria Streptococcus sanguinis.
There were 5 treatment groups in this study: 10% volatile oils of yellow
betle leaves, boiled water of 35 pieces of yellow betle leaves, emulsifier group,
positive control group (0.12% chlorhexidine gluconate), and the negative control
group (aquadest). The subject was Streptococcus sanguinis obtained from
isolation volunteer supragingival plaque. An amount of 0,1 l bacterial suspension
were then added to the whole tubes and incubated for 18 hours in 37oC with
angulation of 30o. Adhesion of bacteria on the surface of the test tube wall were
measured by spectrophotometer 600 nm. Optical density of the measurement
converted into a percentage of bacterial adherence.
One Way ANOVA test showed significant differences between volatile oils
and boiled water of yellow betle leaves (p<0.05). The conclusion of this study
there was effect of volatile oils and boiled water of yellow betle leaves against the
percentage adherence of the bacteria Streptococcus sanguinis. Ten percent volatile
oils of yellow betle leaves can reduce the percentage adherence of the bacteria
Streptococcus sanguinis better than boiled water of 35 pieces of yellow betle
leaves.
Keyword: yellow betle, volatile oil, boiled water, adhesion, Streptococcus
sanguinis.
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