DEGRADASI SERAT KASAR DAN PROTEIN KASAR PADAJERAMI PADI DENGAN PENAMBAHAN BERBAGAIPROBIOTIK SECARA IN VITRO Oleh: GUSTI MOHAMMAD FAKHRULLOH RIYADHI ( 03910003 ) Animal Husbandry Dibuat: 2008-02-09 , dengan 3 file(s). Keywords: DEGRADASI SERAT KASAR DAN PROTEIN KASAR RINGKASAN GUSTI MOHAMAD FAKHRULLOH RIYADHI, 2007. “DEGRADASI SERAT KASAR DAN PROTEIN KASAR JERAMI PADI DENGAN PENAMBAHAN BERBAGAI PROBIOTIK SECARA IN VITRO” Penelitian ini dilaksanakan pada bulan Agustus sampai dengan September 2007 di Laboratorium Kimia Universitas Muhammadiyah Malang dan Laboratorium Nutrisi Makanan Ternak Universitas Brawijaya Malang. Penelitian ini bertujuan untuk mengetahui kecernaan in vitro selama 48 jam atau degradasi jerami padi dengan penambahan berbagai macam probiotik terhadap kandungan serat kasar dan protein kasar secara in vitro. Materi yang digunakan dalam penelitian ini adalah jerami padi varietas Bramu dan tiga jenis probiotik yaitu mikroorganisme efektif (EM4), starbio dan bio feed. Alat yang digunakan seperangkat alat untuk kecernaan in vitro, dan seperangkat alat analisa proksimat serat kasar dan protein kasar. Metode yang digunakan dalam penelitian ini adalah metode eksperimen dengan menggunakan rancangan percobaan yaitu Rancangan Acak Lengkap menggunakan empat perlakuan dan tiga kali ulangan, adapun variabel yang diukur adalah nilai degradasi serat kasar dan protein kasar. Data yang diperoleh dari Rancangan Acak Lengkap dianalisa dengan analisa variansi, apabila berpengaruh nyata atau sangat nyata dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukkan adanya interaksi yang sangat nyata pada degradasi serat kasar (P<0,01) rata-rata hasil degradasi serat kasar pada perlakuan P0 (kontrol): 39,58%, P1 (jerami padi + EM4): 57,12%, P2 (jerami padi + starbio): 71,1%, P3 (jerami padi + bio feed): 64,5%. Sedangkan nilai degradasi protein kasar tidak berpengaruh nyata (P>0,01) nilai rata-rata pada degradasi protein kasar pada perlakuan P0 (kontrol): 7,71%, P1 (jerami padi + Em4): 9,52%, P2 (jerami Padi + starbio): 10,20%, P3 (jerami padi + bio feed): 9,07%. Perlakuan pemberian probiotik berpengaruh sangat nyata terhadap degradasi serat kasar dan perlakuan terbaik pada jerami yang ditambahkan dengan bio feed dan starbio masing- masing 64,5% dan 71,1%. Penelitian ini disarankan untuk dilanjutkan secara in sacco untuk mengetahui laju degradasi pemberian berbagai level probiotik pada level berapa yang memberikan hasil terbaik. SUMMARY GUSTI MOHAMAD FAKHRULLOH RIYADHI, 2007 “DEGRADATION CRUDE FIBER (CF) AND CRUDE PROTEIN (CP) OF PADDY STRAW WITH ADDITIONS PROBIOTIC BY IN VITRO”. The research was conducted on August to October 2007 in Feed and Nutrition Laboratory Brawijaya University of Malang and Chemistry Laboratory Muhammadiyah University of Malang. The aims of this research was to know 48 hours digestion or degradation of paddy straw with various of probiotic addition which contents of crude fiber and crude protein by in vitro. The material used in this research was paddy straw which kind of Bramu and three types of probiotic were microorganism effective (EM4), starbio and bio feed. Tools that used were set of equipments for degradation in vitro and proximate analysis of crude fiber and crude protein. The design of this research was complete random design with the experime nt method or treatment, which ways four treatment and three times repeating, whereas variable measured was degradation of coarse fiber and coarse protein. Data obtained from complete random design analyzed by variance analysis, if there were very real effect and continued by smallest real different test (BNT). The research result indicate that there were very real effects at degradation of crude fiber (P<0,01) averages the result of degradation crude fiber in treatment P0 (control): 39,58%, P1 (paddy straw + EM4): 57,12%, P2 (paddy straw + starbio): 71,1%, P3 (paddy straw + bio feed): 64,5%. Meanwhile, the value of degradation crude protein was not real effect (P>0,01) value average at degradation of crude protein in treatment P0 (control): 7,71%, P1 (paddy straw + Em4): 9,52%, P2 (paddy straw + starbio): 10,20%, P3 (paddy straw + bio feed): 9,07%. The treatment of giving probiotic have real effects in degradation of crude fiber and the best of treatment in straw which added bio feed and starbio each others 64,5% and 71,1%. The research can be suggested to continuing research by in sacco to know the level of rate degradation giving probiotic to know in which level giving the best results.