laporan akhir hasil penelitian unggulan perguruan tinggi

Bidang Unggulan : Ketahanan Pangan
LAPORAN AKHIR HASIL
PENELITIAN UNGGULAN PERGURUAN TINGGIHIBAH BERSAIING (TAHUN KE-1)
TAHUN ANGGARAN 2012
Judul
:
Mobilisasi 3-Isolat Bakteri Untuk Memacu Proses
Nitrifikasi Pada Budidaya Tambah Udang
Ketua
:
Dr. Ir. Anik. M. Ariati, MSc
Anggota
:
1. Ir. Dewa Gede Raka Wiadnya, M.Sc
2. Ating Yuniarti, S.Pi.m M.Aqua
Dibiayai oleh Direktorat Jenderal Pendidikan Tinggi, Kementerian Pendidikan dan Kebudayaan,
Melalui DIPA Universitas Brawijaya nomor : 0636/023-04.2.16/15/2012, tanggal 9 Desember
2011, dan berdasarkan SK Rektor Universitas Brawijaya Nomor : 058/SK/2012 tanggal 8
Pebruari 2012
LembagaPenelitiandanPengabdiankepadaMasyarakat
UniversitasBrawijaya
2012
1
2
ABSTRAK
Nitrifikasi telah dipelajari secara ekstensif untuk signifikan di dalam siklus
nitrogen.Seringkali penurunan nitrogen dari air limbah yang bermasalah karena laju
pertumbuhan yang rendah dari bakteri yang terlibat. Amobilisasi merupakan metode
yang efisien untuk mempertahankan pertumbuhannya bakteri yang lambat pada reaktor.
Sel-sel amobilreaktor terperangkap dalam gel manik-manik membentuk alternatif
tersebut terpasang-sel sistem. Beberapa teknik untuk evaluasi diberikan, termasuk
tingkat konsumsi substrat dan bahan pendukung (matriks). Bakter ini trifikasi
diamobilisasi dengan alginat, karagenan, polivinil alkohol (PVA) dan serbuk gergaji yang
digunakan sebagai matriks. Reaktor matriks dapat mengoksidasi TAN berkisar 63-78%.
Matrik salginat memiliki efisiensi nitrifikasi sebesar 10-20 persen lebih tinggi dari matriks
lainnya. Penggunaan matriks amobil secara efektif dapat mengoksidasi TAN sampai
tiga.
ABSTRACT
Nitrification has been studied extensively for its significance in the nitrogen cycle. Often
nitrogen removal from wastewater is problematic because of the low growth rate of
thebacteria involved. Immobilization is an efficient method to retain slow-growing
organisms in reactors.Immobilized-cell reactor cells entrapped in gel beadsform an
alternative to these attached-cell systems.Several techniques for evaluation are given,
includingsubstrate consumption rates and support material (matrix).Nitrifying
microorganisms, immobilized by alginate, carrageenan, polyvinyl-alcohol (PVA) and
sawdust were used as a matrix. The matrix reactor removed ranging from 63-78%of
TAN.Alginate matrix have the nitrification efficiency of 10-20 percent higher than other
matrix. The matrix can effectively remove of TAN until three times used.
3
RINGKASAN
Amobilisasi bakteri nitrifikasi dapat menjadi metode yang efektif untuk
menurunkan total amonia-nitrogen (TAN) dari budidaya udang. Hal ini dapat membantu
meningkatkan kualitas air untuk budidaya udang dan engurangi biaya modal dan
operasional.
Tujuan dari penelitian ini adalah untuk mendapatkan matrik penjerap bakteri
nitrifikasi (AOB dan NOB) dalam proses amobilisasi yang mampu menurunkan TAN
yang paling efektif.
Bakteri yang digunakan dalam penelitian ini adalah Nitrosomonas, Nitrobacter
dan Nitrospira. Bakteri ini hasil dari isolasi sedimen tambak udang. Untuk keperluan
amobilisasi, masing-masing bakteri ditumbuhkan pada medianya. Bakteri yang
digunakan untuk proses amobilisasi diambil pada fase ekspon ensial yaitu pada inkubasi
jam ke 12 – 18. Pada penelitian ini menggunakan beberapa matrik seperti alginate,
karagenan, polivnilalkohol (PVA) dan serbuk gergaji sebagai penjerap bakteri. Pengujian
laju nitrifikasi dilakukan dengan sebuah bioreaktor nitrifikasi dengan media yang telah
ditentukan konsentrasi TAN sebesar 1,6mM. Untuk mengetahui efisiensi penggunaan
bakteria mobil, dilakukan pengujian berulang dari manik-manika mobil yang sama.
Hasil penelitian menunjukkan bahwa kemampuan matrik untuk menjerap bakteri
berkisar antara 70-80%. Sedangkan kekuatan gel yang terbentuk paling tinggi pada
matrik alginate dan PVA. Laju penurunan TAN berkisar antara 0,032 sampai 0,042
mM/menit. Laju penurunan TAN sangat dipengaruhi oleh matrik penjerap. Secara ratarata penggunaan penjerap alginate, karagenan, PVA dan serbuk gergaji dapat
menurunkan TAN secara berturut sebesar 0,042; 0,036; 0,033 dan 0,032 mM/menit.
Sedangkan untuk periode penggunaan manik-manika mobil, kemampuan laju
nitrifikasinya menurun secara drastis setelah penggunaan lebih dari tiga kali. Efisiensi
penurunan TAN juga dipengaruhi oleh matrik penjerapnya. Nilai efisiensi penurunan
TAN berkisar antara 62 sampai 78%, dengan efisiensi paling tinggi pada matrik alginat.
Sedangkan efisiensinya menurun sampai 50% setelah penggunaan lebih dari tiga kali.
Dari hasil ini dapat disimpulkan bahwa: Alginat, karagenan, PVA dan serbuk
gergaji merupakan bahan yang dapat digunakan sebagai matrik penjerap untuk bakter
initrifikasi. Kemampuan nitrifikasi yang tertinggi dicapai pada penggunaan matrik
penjerap alginate. Matrik penjerap alginate mempunyai efisiensi nitrifikasi 10-20 persen
lebih tinggi dibanding dengan penjerap karagenan, PVA dan serbuk gergaji. Sehingga
dalam penelitian lanjutan dapat disarankan bahwa Alginat relative efektif dalam
nitrifikasi, tetapi harganya cukup mahal. Sebaliknya serbuk gergaji relative murah
dengan kemampuan 20% lebih rendah. Kedua jenis penjerap ini perlu diuji kemampuan
nitrifikasinya pada budidaya udang.
4
SUMMARY
Immobilization of nitrifying bacteria can be an effective method to reduce the total
ammonia-nitrogen (TAN) from shrimp farming. This can help improve the quality of water
for shrimp farming and reduce capital and operational costs.
The purpose of this study was to obtain a matrix entrapment nitrifying bacteria
(AOB and NOB) in animmobilization process that can effectively reduce of the TAN
concentration.
The bacteria used in this study wereNitrosomonas, Nitrobacter and Nitrospira.
This bacteriaare the result of the isolation from the shrimp pond sediment. For the
purposes of immobilization, each bacterium was grown in the specific medium. The
bacteria that are used toimmobile processtaken from the exponential growth phase at 12
to 18 hours incubation time.Several metrics such as alginate, carrageenan, polyvinyl
alcohol (PVA) and sawdust were used as bacteria entrapped. Nitrification rate was
conducted by using of nitrification bioreactor with media specified TAN concentration of
1.6 mM. To determine the efficiency of the use of immobilized bacteria, repeated testing
of same immobilized beads was applied.
The results showed that the ability of the matrix to adsorb bacteria ranged from
70-80%. While the strength of the gel formed highest in alginate and PVA matrix. The
removal rate of TAN ranged from 0.032 to 0.042 mM/min. The TAN removal rate is
influenced by the matrix entrapped. The matrix of alginate, carrageenan, PVA and
sawdust can removed TAN at 0.042; 0.036; 0.033 and 0.032 mM/min, respectively.
While for the use of immobilized beads, the ability of nitrification rate decreases
dramatically after the use of more than three times. TAN removal efficiency is also
affected by the type of matrix. TAN removal efficiency values ranged from 62 to 78%,
with the highest efficiency in the alginate matrix. While the removal efficiency decreased
to 50% after the use of more than three times.
From these results it can be concluded that: Alginate, carrageenan, PVA and
sawdust are a material that can be used as a matrix for bacterial immobilization. The
ability of nitrification of the highest achieved in the use of alginate matrix. Alginate matrix
have the nitrification efficiency of 10-20 percent higher than matrix of carrageenan, PVA
and sawdust. Thus, in further research can be suggested that Alginate relatively
effective in nitrification, but the price is quite expensive. In contrast with the relatively low
cost sawdust 20% lower capacity. Both types of matrix need to be tested for their
nitrification on shrimp culture
5
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