xi INTISARI PROFIL DARAH TIKUS PUTIH (Rattus norvegicus

INTISARI
PROFIL DARAH TIKUS PUTIH (Rattus norvegicus) WISTAR
PENDERITA DIABETES MELLITUS INDUKSI STREPTOZOTOCIN
DIAN MUSLIMAH
11/312545/KH/07039
Penelitian ini bertujuan mengetahui efek pemberian streptozotocin
terhadap kadar glukosa darah, gambaran eritrosit dan leukosit pada tikus penderita
diabetes mellitus buatan.
Dalam penelitian digunakan 19 ekor tikus jantan galur Wistar umur dua
bulan dengan berat badan 170-250 gram. Tikus percobaan dibagi dua kelompok
secara acak, kelompok kontrol terdiri dari 5 ekor tikus, kelompok perlakuan terdiri
dari 14 ekor tikus. Tikus-tikus diadaptasikan selama satu minggu. Pada hari ke-1
kelompok kontrol tidak diberikan perlakuan, kelompok perlakuan diinduksi
Streptozotocin dosis 40 mg/kg BB/IP. Pengambilan darah dilakukan sebelum
tikus diinjeksi Streptozotocin (hari ke-0), setelah diinjeksi Streptozotocin (Hari
ke-4, Hari ke-11, Hari ke-26) dan diakhir penelitian (hari ke-74). Parameter yang
diukur adalah kadar glukosa darah, gambaran eritrosit (total eritrosit, kadar
hemoglobin, MCV, MCH, MCHC), nilai PCV, nilai TPP dan jumlah total
leukosit.
Hasil penelitian menunjukkan gejala klinis yang terlihat polyuria,
polidipsi, polifagi, dan kekurusan. Kadar glukosa hari ke-4, ke-11, ke-26 dan hari
ke-74 menunjukkan ada perbedaan signifikan (P<0,05) pada tikus kelompok
perlakuan (>500 mg/100ml). Hasil pemeriksaan total eritrosit, kadar hemoglobin,
MCV, MCH, MCHC, PCV, TPP dan jumlah total leukosit hari ke-4, ke-11, ke-26,
hari ke-74 menunjukkan tidak ada perbedaan signifikan (P>0,05) antara tikus
kelompok kontrol dan perlakuan. Induksi Streptozotocin dosis tunggal 40 mg/kg
BB/IP mampu menginduksi diabetes mellitus namun tidak berpengaruh terhadap
gambaran eritrosit (total eritrosit, kadar hemoglobin, MCV, MCH, MCHC, nilai
PCV), nilai TPP dan jumlah total leukosit.
Kata Kunci : diabetes mellitus, streptozotocin, hematologi, kadar glukosa, tikus
wistar jantan
xi
WISTAR WHITE RATS (Rattus norvegicus) IN DIABETES MELLITUS
BLOOD PROFILE INDUCED BY STREPTOZOTOCIN
DIAN MUSLIMAH
11/312545 / KH / 07039
ABSTRACT
This research is aim to determine the effects of streptozotocin administration on
blood glucose levels and erythrocyte and leukocyte profile in rats with diabetes
mellitus artificial.
The research used 19 male Wistar rats two months, 170-250 grams. Rats were
divided into two groups randomly, the control group consisted of 5 rats, the
treatment group consisted of 14 rats. They were kept in practical animal
laboratorium FKH UGM with food and water free access, after being adapted for
1 week, Streptozotocin 40 mg/kg BW were injected on day 1 to treatment rats
group. Group A did not give treatment, group B was injected by Streptozotocin 40
mg/kb BW/IP. Blood sampling had been taken on day 0, after injection of
Streptozotocin (Day 4, Day 11, Day 26) and the end of research (day 74).
Parameters measured were blood glucose levels, erythrocyte profile (total
erythrocytes, hemoglobin level, MCV, MCH, MCHC), PCV value, the value of
TPP and total of leukocytes.
The results showed that glucose levels on day 4, 11th, 26th and 74th day showed
no significant difference (P <0.05) in rat treated groups. The results of the
examination of erythrocyte profile (total erythrocytes, hemoglobin level, MCV,
MCH, MCHC), PCV value, the value of TPP and overview of leukocytes on day
4, 11th, 26th and 74th day showed no significant difference (P> 0.05) in the
control group and treated rats. Streptozotocin induction dose of 40 mg/kg BB/IP is
able to host diabetes but does not affect the picture erythrocytes (total
erythrocytes, hemoglobin level, MCV, MCH, MCHC), PCV value, the value of
TPP and total of leukocytes.
Keywords: diabetes mellitus, streptozotocin, total eritosit, hematology, glucose
levels, male wistar rat.
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