PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS

PROBIOTIK BAKTERI ASAM LAKTAT INDIGENOUS PENGARUHNYA
TERHADAP BIOEFISIENSI DAN RESPON IMUN PUYUH JANTAN
INTISARI
Sapta Chandra Marnadi Hidayat
12/342668/PPT/00820
Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi probiotik bakteri
asam laktat (BAL) indigenous terhadap bioefisiensi dan respon imun pada puyuh jantan.
Probiotik BAL indigenous yang digunakan terdiri dari campuran tiga strain, yaitu
Lactobacillus murinus (Ar3), Streptococcus thermophilus (Kp2), dan Pediococcus
acidilactici (Kd6). Sebanyak 192 ekor day old quail (DOQ) puyuh jantan dari Hatchery PT.
Peksi Gunaraharja dibagi secara acak dalam dua kelompok perlakuan vaksin dan nonvaksin dengan empat level suplementasi probiotik BAL dipelihara selama 42 hari.
Kelompok perlakuan vaksin pada penelitian ini yaitu (T1) tanpa suplementasi probiotik
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BAL, (T2) suplementasi probiotik BAL 10 , (T3) suplementasi probiotik BAL 10 , (T4)
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suplementasi probiotik BAL 10 CFU/mL/ekor/hari. Kelompok perlakuan non-vaksin yaitu
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(T5) tanpa suplementasi probiotik BAL, (T6) suplementasi probiotik BAL 10 , (T7)
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suplementasi probiotik BAL 10 , (T8) suplementasi probiotik BAL 10 CFU/mL/ekor/hari.
Suplementasi probiotik BAL melalui tetes mulut sebanyak 1 mL diberikan mulai hari ke
14 – 42. Vaksin yang digunakan untuk kelompok perlakuan vaksin adalah ND Hitcner B1
aktif dan ND La Sota aktif melaui tetes mata masing-masing pada hari ke 4 dan 28.
Semua perlakuan pada hari ke 36 diinjeksi 0,1 mL SRBC 5 %. Sampel darah diambil 6
hari kemudian untuk uji hemaglutinasi assay (HA). Pakan dan air minum diberikan secara
ad libitum selama pemeliharaan. Pakan yang digunakan sesuai dengan standar
kebutuhan puyuh (Protein kasar 24,03% dan ME 2908,05 kcal/kg). Data dianalisis
dengan analisis variansi RAL pola faktorial 2 x 4 dilanjutkan dengan uji multiple
comparison Tukey-HSD dan uji kontras polinomial orthogonal. Hasil penelitian ini pada
perlakuan vaksinasi berpengaruh terhadap bioefisensi dan respon imun puyuh (p<0,05)
meliputi tinggi villi pada ileum dan sekum masing-masing 249±23 dan 122,3±25; lebar villi
pada jejunum, ileum, dan sekum masing-masing 116,1±25,4, 97,1±14,9, dan 57,1±10,8;
kedalaman kripta pada duodenum, jejunum, ileum, dan sekum masing-masing 76±15,4,
59,3±8,7, 56,3±13,5, dan 36±7,8 (µm); konsumsi pakan 471,2±14,6 (g/ekor); konversi
pakan 3,97±0,39; dan berat bursa fabricius 0,196±0,1 (g). Perlakuan probiotik BAL
berpengaruh terhadap bioefisensi puyuh (P<0,05) meliputi tinggi villi pada duodenum,
jejunum, ileum, dan sekum masing-masing 352,5±40, 326,1±40, 5257,8±12,9,
120,7±42,1; lebar villi pada semua segmen masing-masing 139,1±27,8, 141±15,1,
103±9,6, 60,8±12,2; kedalaman kripta pada semua segmen masing-masing 84,9±13,9,
63,4±6,6, 61,3±6,4, 38,5±9,5 (µm); pertambahan bobot badan 115,6±4,9 (g), dan
konversi pakan 3,84±0,24. Terdapat interaksi antara perlakuan vaksinasi dengan
probiotik BAL (P<0,05) terhadap histomorfologi pada semua segmen usus. Berdasarkan
penelitian yang telah dilakukan dapat diambil kesimpulan bahwa perlakuan vaksinasi
dapat meningkatkan bioefisiensi puyuh dan respon imun, sedangkan suplementasi
probiotik BAL dapat meningkatkan bioefisiensi puyuh. Interaksi antara perlakuan
vaksinasi dan probiotik BAL berpengaruh terhadap histomorfologi usus.
Kata kunci: Probiotik BAL, Bioefisiensi, Respon imun, Puyuh jantan
EFFECTS OF PROBIOTIC LACTIC ACID BACTERIA ON
BIOEFFICIENCY AND IMMUNE RESPONSE OF
MALE JAPANESE QUAILS
ABSTRACT
Sapta Chandra Marnadi Hidayat
12/342668/PPT/00820
The present study was conducted to investigate the effects of supplementation
probiotic lactic acid bacteria (LAB) indigenous on bioefficiency and immune response of
male japanese quail. The probiotic LAB indigenous is consisting of three strains
Lactobacillus murinus (Ar3), Streptococcus thermophilus (KP2), and Pediococcus
acidilactici (Kd6). A total of 192 day-old male quails (DOQ) from PT. Peksi Gunaraharja
Hatchery were randomly divided into two treatment groups the vaccine and nonvaccine
with four levels of supplementation of probiotic LAB raising for 42 days. Vaccine
treatment group in this study is consisting of (T1) without supplementation of probiotic
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LAB, (T2) supplementation of probiotic LAB 10 , (T3) supplementation of probiotic LAB
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10 , (T4) supplementation of probiotic LAB 10 CFU/mL/bird/day. The non-vaccine
treatment group is consisting of (T5) without supplementation of probiotic LAB, (T6) of
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probiotic supplementation BAL 10 , (T7) supplementation of probiotic LAB 10 , (T8)
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probiotic supplementation BAL 10 CFU/mL/bird/day. Probiotic LAB supplementation via
oral drops of 1 mL given from day 14 – 42.The vaccine treatment group will be vaccineted
with ND Hitcner active B1 and ND La Sota inactive via eye drops on days 4 and 28. All
treatments at day 36 will be injected with 0.1 mL of 5% SRBC. Blood samples were
taken six days later for hemagglutination assay (HA) test. Feed and water were given ad
libitum during raising. Feed used in accordance with the standard of quail diet (24.03 %
CP and 2908.05 kcal/kg ME). Data were analyzed by analysis of variance CRD factorial
2 x 4 continued with multiple comparison test of Tukey-HSD test and orthogonal
polynomial contrasts. Results of this research on vaccination treatment affected
bioefficiency and immune response of quail (p <0.05) include villi height in the ileum and
cecum respectively 249 ± 23 and 122.3 ± 25; villi width in the jejunum, ileum and cecum
respectively 116.1 ± 25.4, 97.1 ± 14.9 and 57.1 ± 10.8; crypt depth in the duodenum,
jejunum, ileum and cecum of each 76 ± 15.4, 59.3 ± 8.7, 56.3 ± 13.5 and 36 ± 7.8 (µm);
feed consumption of 471.2 ± 14.6 (g/bird); feed conversion 3.97 ± 0.39; and bursa of
fabricius weight 0.196 ± 0.1 (g). LAB probiotic treatment affected on quail bioefficiency
(P <0.05) include villi height in the duodenum, jejunum, ileum and cecum respectively
352.5 ± 40, ± 40 326.1, 5257.8 ± 12.9, 120 7 ± 42.1; villi width for all segments
respectively 139.1 ± 27.8, 15.1 ± 141, 103 ± 9.6, 60.8 ± 12.2; crypt depth in all segments
respectively 84.9 ± 13.9, 63.4 ± 6.6, 61.3 ± 6.4, 38.5 ± 9.5 (µm); body weight 115.6 ± 4.9
(g) and feed conversion 3.84 ± 0.24. There is interaction between vaccination and
probiotic LAB (P <0.05) against histomorfology on all segments of the intestinal. Based
on research that has been done can be concluded that the vaccination treatment can
improved bioefficiency and immune response of quail, whereas LAB probiotic
supplementation can improved quail bioefficiency. The interaction treatment between
vaccination and LAB probiotic supplementation affected intestinal histomorfology.
Keywords: Probiotics LAB, Bioefficiency, Immune response, Male quail